PA5-52629

antibody from Invitrogen Antibodies

Targeting: HEXIM1 CLP-1, EDG1, HIS1, MAQ1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-52629

Provider

Invitrogen Antibodies

Product name

HEXIM1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: AKSDDTSDDD FMEEGGEEDG GSDGMGGDGS EFLQRDFSET YERYHTESLQ NMSKQELIKE YLELEKCLSR MEDENNRLRL ESKRLGGDDA RVRELELELD RLRAENLQLL TENELHRQQE RA Highest antigen sequence identity to the following orthologs: Mouse - 95%, Rat - 94%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.4 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of HEXIM1 in human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a HEXIM1 Polyclonal Antibody (Product # PA5-52629).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HEXIM1 was performed using A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HEXIM1 Polyclonal Antibody (Product # PA5-52629) at 2 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification..

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of binding of endogenous HEXIM1 protein to specific RNA using Anti-HEXIM1 Antibody: RNA Immunoprecipitation (RIP) was performed using Anti-HEXIM1 Recombinant Rabbit Polyclonal Antibody (Product # PA5-52629, 5 µg) on whole cell lysate from 2 million HCT 116 cells. Normal Rabbit IgG was used as a negative IP control. RNA purified by RiboPure™ RNA Purification Kit (Product # AM1924) was analyzed by RT-PCR using the Power SYBR® Green RNA-to-CT™ 1-Step Kit (Product # 4389986) with RIP primer pairs over 7SK, ACTB mRNA, U1 snRNA and 18s rRNA. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.