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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-28807 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- REXO4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: H1299, HeLaS3. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.18 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of XPMC2H using 30 µg of A) H1299 and B) HeLa S3 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a XPMC2H polyclonal antibody (Product # PA5-28807) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using REXO4 Polyclonal Antibody (Product # PA5-28807). Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with REXO4 Polyclonal Antibody (Product # PA5-28807) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of XPMC2H in paraformaldehyde-fixed HeLa cells using a XPMC2H polyclonal antibody (Product # PA5-28807) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- REXO4 Polyclonal Antibody detects REXO4 protein at nucleus on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. REXO4 Polyclonal Antibody (Product # PA5-28807) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
