Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [5]
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- Product number
- NBP2-32438 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Nicotinamide N-Methyltransferase/NNMT Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Nicotinamide N-Methyltransferase/NNMT antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP2-32438] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP2-32438] - Analysis in human cell lines U2OS and SK-MEL-30 using anti-NNMT antibody. Corresponding NNMT RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP2-32438] - Staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP2-32438] - Staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP2-32438] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP2-32438] - Staining of human pancreas shows moderate to strong granular cytoplasmic positivity in exocrine glandular cells.
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nicotinamide N-Methyltransferase/NNMT Antibody [NBP2-32438] - Staining of human skin shows no positivity in squamous epithelial cells as expected.