15123-1-AP

antibody from Proteintech Group

Targeting: NNMT

Provider product page for 15123-1-AP

Western blot

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Immunocytochemistry

Immunoprecipitation

Antibody data

Antibody Data
Antigen structure
References [31]
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Validations
Western blot [1]

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Product number: 15123-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#15123-1-AP, RRID:AB_2153579
Product name: NNMT antibody
Antibody type: Polyclonal
Description: KD/KO validated NNMT antibody (Cat. #15123-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

NNMT protein structure - 15123-1-AP shown in red.

Submitted references 1-methylnicotinamide attenuated inflammation and regulated flora in Necrotizing enterocolitis.

Hou L, Du J, Li J, Luo Q, Kang Q
PloS one 2025;20(6):e0324068

SNORA13 antisense oligonucleotides enhances the therapeutical effects of 5-fluorouracil in colon adenocarcinoma.

Wang Y, An H, Zhang Y, Lyu QR, Zhang Z
Frontiers in pharmacology 2025;16:1564682

NNMT inhibition in cancer-associated fibroblasts restores antitumour immunity.

Heide J, Bilecz AJ, Patnaik S, Allega MF, Donle L, Yang K, Teich E, Li Y, Lin Q, Kong K, Liu L, Yang TG, Cheng KC, Shrimp JH, Hanson QM, Shen M, Sun H, Shah H, Schweizer L, Zawieracz K, Olland A, White A, Suto RK, Alhunayan R, Taşdemir M, Longman N, Liang H, Mann M, Stott GM, Hall MD, Schwörer S, Weichselbaum RR, Piffkó A, Lengyel E
Nature 2025 Sep;645(8082):1051-1059

NNMT-mediated N(6)-methyladenosine modification of NR4A3 mRNA facilitates lymphatic metastasis of gastric adenocarcinoma.

Zhao X, Bao L, Qian Y, Zhou S, Chen C, Ma Z, Xu J, Li M, Niu W, Wang B, Wang Q, Wang Z, Wang S, Wang M
Oncogene 2025 Sep;44(33):2970-2982

Liver metastasis or peritoneal metastasis: single-cell RNA sequencing reveals the organotropism in colorectal cancer is driven by distinct partial-EMT processes.

Yu C, Lu W, Wu J, Fang X, Wang X, Zhang G, Chen S, Mei Y, Chen H, Ye F, Yang H, Qian Y, Kong X, Zhang P, Wang J, Guo G, Ding K
Cancer letters 2025 Oct 1;629:217880

NAD Metabolism Regulates Proliferation of Macrophages in Atherosclerosis.

Sinha SK, Swichkow CR, Farahi L, Péterfy M, Pan C, Zhou Z, Seldin MM, Mack JJ, Davis RC, Shih DM, Lusis AJ
Arteriosclerosis, thrombosis, and vascular biology 2025 Nov;45(11):1997-2014

PRDX6 Prevents NNMT Ubiquitination and Degradation as a Nonenzymatic Mechanism to Promote Ovarian Cancer Progression.

Wu X, Luo L, Wang M, Dong L, Fan J, Zeng Y, Li S, Wang K
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Mar;12(12):e2416484

Prognostic and Therapeutic Value of Metabolism-Related Genes in Nephroblastoma: A Focus on the Key Gene NNMT and Its Regulative Effect on Metabolism.

Ding C, Huang F, Gao H, Zhang W, Lu Z, Zhang L, Zhang B, Li D, Li Y, Bi D, Sun F
Cell biochemistry and function 2025 Jul;43(7):e70097

Downregulation of ferroptosis-related ATF3 alleviates lupus nephritis progression.

Xiao G, Tang S, Zhang Y, Yuan Q, Sun D, Wang W
Gene 2025 Jan 20;934:149009

Inhibition of NNMT enhances drug sensitivity in lung cancer cells through mediation of autophagy.

Wang J, Zhang M, You X, Xu Y, Zhang C, Li Y, Yang C, Wang Q
Frontiers in pharmacology 2024;15:1415310

Pan-cancer analysis combined with experiments predicts NNMT as a therapeutic target for human cancers.

Huang H, Su L, Zhang R, Wu D, Ding C, Chen C, Zhu G, Cao P, Li X, Li Y, Liu H, Chen J
Discover oncology 2024 May 29;15(1):196

Single-cell analysis reveals insights into epithelial abnormalities in ovarian endometriosis.

Yan J, Zhou L, Liu M, Zhu H, Zhang X, Cai E, Xu X, Chen T, Cheng H, Liu J, Wang S, Dai L, Chang X, Tang F
Cell reports 2024 Mar 26;43(3):113716

Time-restricted feeding ameliorates non-alcoholic fatty liver disease through modulating hepatic nicotinamide metabolism via gut microbiota remodeling.

Feng R, Yang W, Feng W, Huang X, Cen M, Peng G, Wu W, Wang Z, Jing Y, Long T, Liu Y, Li Z, Chang G, Huang K
Gut microbes 2024 Jan-Dec;16(1):2390164

Comparative Analysis of Two NNMT Bisubstrate Inhibitors through Chemoproteomic Studies: Uncovering the Role of Unconventional SAM Analogue Moiety for Improved Selectivity.

Meng Y, Iyamu ID, Ahmed NAM, Huang R
ACS chemical biology 2024 Jan 19;19(1):89-100

Control of NAD(+) homeostasis by autophagic flux modulates mitochondrial and cardiac function.

Zhang Q, Li Z, Li Q, Trammell SA, Schmidt MS, Pires KM, Cai J, Zhang Y, Kenny H, Boudina S, Brenner C, Abel ED
The EMBO journal 2024 Feb;43(3):362-390

Knockdown of nicotinamide N-methyltransferase ameliorates renal fibrosis caused by ischemia-reperfusion injury and remodels sphingosine metabolism.

Xu W, Hou L
Clinical and experimental nephrology 2024 Dec;28(12):1241-1253

Identifying polyamine related biomarkers in diagnosis and treatment of ulcerative colitis by integrating bulk and single-cell sequencing data.

Wei W, Lu Y, Zhang M, Guo J, Zhang H
Scientific reports 2024 Aug 5;14(1):18094

Structure-Activity Relationship Studies on Cell-Potent Nicotinamide N-Methyltransferase Bisubstrate Inhibitors.

Iyamu ID, Zhao T, Huang R
Journal of medicinal chemistry 2023 Aug 10;66(15):10510-10527

Stromal nicotinamide N-methyltransferase orchestrates the crosstalk between fibroblasts and tumour cells in oral squamous cell carcinoma: evidence from patient-derived assembled organoids.

Zhao H, Li R, Chen Y, Yang X, Shang Z
Oncogene 2023 Apr;42(15):1166-1180

Nicotinamide N-Methyltransferase Remodeled Cell Metabolism and Aggravated Proinflammatory Responses by Activating STAT3/IL1β/PGE(2) Pathway.

Yang C, Wang T, Zhu S, Zong Z, Luo C, Zhao Y, Liu J, Li T, Liu X, Liu C, Deng H
ACS omega 2022 Oct 25;7(42):37509-37519

NNMT-DNMT1 Axis is Essential for Maintaining Cancer Cell Sensitivity to Oxidative Phosphorylation Inhibition.

Wu C, Liu Y, Liu W, Zou T, Lu S, Zhu C, He L, Chen J, Fang L, Zou L, Wang P, Fan L, Wang H, You H, Chen J, Fang JY, Jiang C, Shi Y
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2022 Nov 16;10(1):e2202642

Effect of nicotinamide N-methyltransferase on lipid accumulation in 3T3-L1 adipocytes.

Xu W, Hou L, Li P, Li L
Bioengineered 2022 May;13(5):12421-12434

Disrupted liver oxidative metabolism in glycine N-methyltransferase-deficient mice is mitigated by dietary methionine restriction.

Rome FI, Hughey CC
Molecular metabolism 2022 Apr;58:101452

The significance of NAD + metabolites and nicotinamide N-methyltransferase in chronic kidney disease.

Takahashi R, Kanda T, Komatsu M, Itoh T, Minakuchi H, Urai H, Kuroita T, Shigaki S, Tsukamoto T, Higuchi N, Ikeda M, Yamanaka R, Yoshimura N, Ono T, Yukioka H, Hasegawa K, Tokuyama H, Wakino S, Itoh H
Scientific reports 2022 Apr 16;12(1):6398

[Expression of nicotinamide-N-methyltransferase in gastric cancer and its biological and clinicopathological significance].

Wen Z, Liang W, Zhong Y, Sun F, Zhang Q
Nan fang yi ke da xue xue bao = Journal of Southern Medical University 2021 Jun 20;41(6):828-838

Glucose availability regulates nicotinamide N-methyltransferase expression in adipocytes.

Ehebauer F, Ghavampour S, Kraus D
Life sciences 2020 May 1;248:117474

Elevated N-methyltransferase expression induced by hepatic stellate cells contributes to the metastasis of hepatocellular carcinoma via regulation of the CD44v3 isoform.

Li J, You S, Zhang S, Hu Q, Wang F, Chi X, Zhao W, Xie C, Zhang C, Yu Y, Liu J, Zhao Y, Liu P, Zhang Y, Wei X, Li Q, Wang X, Yin Z
Molecular oncology 2019 Sep;13(9):1993-2009

Downregulation of nicotinamide N-methyltransferase inhibits migration and epithelial-mesenchymal transition of esophageal squamous cell carcinoma via Wnt/β-catenin pathway.

Cui Y, Zhang L, Wang W, Ma S, Liu H, Zang X, Zhang Y, Guan F
Molecular and cellular biochemistry 2019 Oct;460(1-2):93-103

Nicotinamide N-Methyltransferase and Its Precursor Substrate Methionine Directly and Indirectly Control Malignant Metabolism During Progression of Renal Cell Carcinoma.

Holstein S, Venz S, Junker H, Walther R, Stope MB, Zimmermann U
Anticancer research 2019 Oct;39(10):5427-5436

Dysregulated transmethylation leading to hepatocellular carcinoma compromises redox homeostasis and glucose formation.

Hughey CC, James FD, Wang Z, Goelzer M, Wasserman DH
Molecular metabolism 2019 May;23:1-13

Persistent Exposure to Porphyromonas gingivalis Promotes Proliferative and Invasion Capabilities, and Tumorigenic Properties of Human Immortalized Oral Epithelial Cells.

Geng F, Liu J, Guo Y, Li C, Wang H, Wang H, Zhao H, Pan Y
Frontiers in cellular and infection microbiology 2017;7:57

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The NNMT antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human NNMT. This antibody recognizes human, mouse, rat antigen. The NNMT antibody has been validated for the following applications: ELISA, WB analysis.
Appendix: 15123-1-AP.pdf

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