Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-51252 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NNMT Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The antibody detects endogenous levels of total NNMT protein.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Nnmt was performed by loading Lane 1-3: Hepg2 cell, mouse lung tissue and HT-29 cell lysates (40µg) on to a 12% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a Nnmt antibody (Product # PA5-51252) at a 1/400 dilution for 10 seconds, followed by Goat anti rabbit IgG at 1/8000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Nnmt was performed by loading Lane 1-3: Hepg2 cell, mouse lung tissue and HT-29 cell lysates (40µg) on to a 12% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a Nnmt antibody (Product # PA5-51252) at a 1/400 dilution for 10 seconds, followed by Goat anti rabbit IgG at 1/8000 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NNMT in paraffin-embedded Human breast cancer tissue. Samples were probed using a NNMT polyclonal antibody (Product # PA5-51252) at a dilution of 1/20.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NNMT in paraffin-embedded Human breast cancer tissue. Samples were probed using a NNMT polyclonal antibody (Product # PA5-51252) at a dilution of 1/20.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NNMT in paraffin embedded Human breast cancer tissue using NNMT Polyclonal Antibody (Product # PA5-51252) at a 1:100 dilution.