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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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- Product number
- H00004837-M03 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00004837-M03, RRID:AB_733231
- Product name
- NNMT monoclonal antibody (M03), clone 2F2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant NNMT.
- Antigen sequence
MESGFTSKDTYLSHFNPRDYLEKYYKFGSRHSAES
QILKHLLKNLFKIFCLDGVKGDLLIDIGSGPTIYQ
LLSACESFKEIVVTDYSDQNLQELEKWLKKEPEAF
DWSPVVTYVCDLEGNRVKGPEKEEKLRQAVKQVLK
CDVTQSQPLGAVPLPPADCVLSTLCLDAACPDLPT
YCRALRNLGSLLKPGGFLVIMDALKSSYYMIGEQK
FSSLPLGREAVEAAVKEAGYTIEWFEVISQSYSST
MANNEGLFSLVARKLSRPL- Isotype
- IgG
- Antibody clone number
- 2F2
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of NNMT expression in transfected 293T cell line by NNMT monoclonal antibody (M03), clone 2F2.Lane 1: NNMT transfected lysate(29.6 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged NNMT is approximately 1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of NNMT transfected lysate using anti-NNMT monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with NNMT MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
