500-P245BT-1MG

antibody from Invitrogen Antibodies

Targeting: ARTN ENOVIN, EVN, NBN

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

500-P245BT-1MG

Provider

Invitrogen Antibodies

Product name

Artemin Polyclonal Antibody, Biotin, PeproTech®

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

AA Sequence of recombinant protein: AGGPGSRARA AGARGCRLRS QLVPVRALGL GHRSDELVRF RFCSGSCRRA RSPHDLSLAS LLGAGALRPP PGSRPVSQPC CRPTRYEAVS FMDVNSTWRT VDRLSATACG CLG. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Artemin. Anti-Human Artemin-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hArtemin by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Artemin (500-P245) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hArtemin. Western Blot: To detect hArtemin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hArtemin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P245BT-1MG will be provided as 2 x 500 µg

Reactivity

Human

Host

Rabbit

Conjugate

Biotin

Vial size

2 x 500 µg

Concentration

0.1-1.0 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot: To detect hArtemin by Western Blot analysis Artemin Polyclonal Antibody, Biotin (Product # 500-P245BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hArtemin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Conjugate

Biotin

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sandwich ELISA: To detect hArtemin by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of Artemin Polyclonal Antibody, Biotin (Product # 500-P245BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Artemin Polyclonal Antibody (Product # 500-P245-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hArtemin.

Conjugate

Biotin