Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- LS-B79 - Provider product page
- Provider
- LSBio
- Proper citation
- LifeSpan Cat#LS-B79, RRID:AB_2073621
- Product name
- IHC-plus™ CCNL1 / Cyclin L1 Antibody (aa314-369) LS-B79
- Antibody type
- Polyclonal
- Description
- Protein A purified
- Reactivity
- Human
- Host
- Rabbit
- Storage
- Store at -20°C. Aliquot to avoid freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of Protein A Purified anti-Cyclin L1a antibody shows detection of a band ~59 kDa corresponding to a Cyclin L1a (arrowhead) present in mouse brain whole cell lysate. Approximately 35g of lysate was separated by 4-20% SDS-PAGE followed by transfer to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:3,500 for 2h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.