PA5-36070

antibody from Invitrogen Antibodies

Targeting: CCNL1 ania-6a

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-36070

Provider

Invitrogen Antibodies

Product name

Cyclin L1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody detects endogenous protein at a molecular weight of 60 kDa. Purity is >95% by SDS-PAGE.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

MiR-5195-3p functions as a tumor suppressor in prostate cancer via targeting CCNL1.

Zeng X, Hu Z, Shen Y, Wei X, Gan J, Liu Z
Cellular & molecular biology letters 2022 Mar 8;27(1):25

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MiR-5195-3p suppressed cell proliferation and cell cycle G1/S transition through CCNL1 mediation. PC-3 cells were transfected with si-CCNL1 or si-NC, as well as co-transfection with miR-5195-3p mimics and pcDNA3.1-CCNL1. A Western blot was used to determine the Cyclin L1 protein expression levels in the above transfected PC-3 cells. B Cell proliferation was tested with CCK-8 assay in the above transfected PC-3 cells. C - D Cell cycle distribution was determined by PI staining and flow cytometry analysis in the above transfected PC-3 cells. Data are presented as mean +- standard deviation. * p < 0.05, ** p < 0.01, *** p < 0.001 compared with si-NC or miR-5195-3p mimics

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MiR-5195-3p induced the regression of prostate tumorigenesis in vivo. A MiR-5195-3p expression in stable miR-5195-3p mimics or miR-NC overexpression PC-3 cells determined by quantitative real-time PCR. B Image of tumor xenografts in nude mice injected subcutaneously with miR-5195-3p-overexpressing PC-3 cells. C Tumor volume was measured every 5 days. D Changes in the tumor weight in mice after miR-5195-3p overexpression. E MiR-5195-3p expression in tumor tissue isolated from miR-5195-3p mimics and miR-NC groups of nude BALB/c mice. F Protein expression levels of Cyclin L1, CDK4, and Cyclin D1 expression were determined by western blot analysis. Data are presented as mean +- standard deviation. ** p < 0.01, *** p < 0.001 compared with miR-NC