12922-3-AP

antibody from Proteintech Group

Targeting: HDAC2 KDAC2, RPD3, YAF1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

12922-3-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#12922-3-AP, RRID:AB_2118516

Product name

HDAC2 antibody

Antibody type

Polyclonal

Description

KD/KO validated HDAC2 antibody (Cat. #12922-3-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Bisphenol-A exposure leads to neurotoxicity through upregulating the expression of histone deacetylase 2 in vivo and in vitro.

Bi N, Gu X, Fan A, Li D, Wang M, Zhou R, Sun QC, Wang HL
Toxicology 2022 Jan 15;465:153052

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Multi-omics analyses reveal the mechanisms of Arsenic-induced male reproductive toxicity in mice.

Peng Z, Yang Q, Yeerken R, Chen J, Liu X, Li X
Journal of hazardous materials 2022 Feb 15;424(Pt C):127548

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Epigenetic derepression converts PPARγ into a druggable target in triple-negative and endocrine-resistant breast cancers.

Loo SY, Syn NL, Koh AP, Teng JC, Deivasigamani A, Tan TZ, Thike AA, Vali S, Kapoor S, Wang X, Wang JW, Tan PH, Yip GW, Sethi G, Huang RY, Hui KM, Wang L, Goh BC, Kumar AP
Cell death discovery 2021 Sep 27;7(1):265

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MIER3 induces epithelial-mesenchymal transition and promotes breast cancer cell aggressiveness via forming a co-repressor complex with HDAC1/HDAC2/Snail.

Huang W, Chen J, Liu X, Liu X, Duan S, Chen L, Liu X, Lan J, Zou Y, Guo D, Zhou J
Experimental cell research 2021 Sep 1;406(1):112722

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New Cortical Neurodegenerative Pathways in the Hypertensive Rat Brain.

Moti BS, Oz E, Olga A, Bella G, Shifra S, Eilam P
Cerebral cortex (New York, N.Y. : 1991) 2021 Oct 22;31(12):5487-5496

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Histone deacetylase HDAC2 silencing prevents endometriosis by activating the HNF4A/ARID1A axis.

Mai H, Liao Y, Luo S, Wei K, Yang F, Shi H
Journal of cellular and molecular medicine 2021 Nov;25(21):9972-9982

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Hepatic COX-2 expression protects mice from an alcohol-high fat diet-induced metabolic disorder by involving protein acetylation related energy metabolism.

Chen M, Sun X, Wei W, Cucarella C, Martín-Sanz P, Casado M, Pi L, Ren B, Cao Q
Alcohol (Fayetteville, N.Y.) 2021 May;92:41-52

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Acetylation of STX17 (syntaxin 17) controls autophagosome maturation.

Shen Q, Shi Y, Liu J, Su H, Huang J, Zhang Y, Peng C, Zhou T, Sun Q, Wan W, Liu W
Autophagy 2021 May;17(5):1157-1169

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KAT8/MOF-Mediated Anti-Cancer Mechanism of Gemcitabine in Human Bladder Cancer Cells.

Zhu H, Wang Y, Wei T, Zhao X, Li F, Li Y, Wang F, Cai Y, Jin J
Biomolecules & therapeutics 2021 Mar 1;29(2):184-194

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ZNF382 controls mouse neuropathic pain via silencer-based epigenetic inhibition of Cxcl13 in DRG neurons.

Ma L, Yu L, Jiang BC, Wang J, Guo X, Huang Y, Ren J, Sun N, Gao DS, Ding H, Lu J, Zhou H, Zou L, Gao Y, Wang L, Sun K, Ming Y, Meng Z, Tao YX, Yan M
The Journal of experimental medicine 2021 Dec 6;218(12)

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Aberrant HDAC3 expression correlates with brain metastasis in breast cancer patients.

Ma L, Qi L, Li S, Yin Q, Liu J, Wang J, She C, Li P, Liu Q, Wang X, Li W
Thoracic cancer 2020 Sep;11(9):2493-2505

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Quantitative analysis of global protein stability rates in tissues.

McClatchy DB, Martínez-Bartolomé S, Gao Y, Lavallée-Adam M, Yates JR 3rd
Scientific reports 2020 Sep 29;10(1):15983

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Mechanisms by which electroacupuncture‑mediated histone acetylation mitigates bone loss in rats with ovariectomy‑induced osteoporosis.

Shu Q, Shao Y, Liu R, Hu Y, Peng Z, Tian J
Molecular medicine reports 2020 Oct;22(4):3453-3463

---

USP38 regulates the stemness and chemoresistance of human colorectal cancer via regulation of HDAC3.

Zhan W, Liao X, Liu J, Tian T, Yu L, Li R
Oncogenesis 2020 May 13;9(5):48

---

Developmental nicotine exposure engenders intergenerational downregulation and aberrant posttranslational modification of cardinal epigenetic factors in the frontal cortices, striata, and hippocampi of adolescent mice.

Buck JM, O'Neill HC, Stitzel JA
Epigenetics & chromatin 2020 Mar 5;13(1):13

---

LukS-PV Inhibits Hepatocellular Carcinoma Progression by Downregulating HDAC2 Expression.

Wang Z, Yu W, Qiang Y, Xu L, Ma F, Ding P, Shi L, Chang W, Mei Y, Ma X
Molecular therapy oncolytics 2020 Jun 26;17:547-561

---

LncRNA NKILA integrates RXFP1/AKT and NF-κB signalling to regulate osteogenesis of mesenchymal stem cells.

Zhang Y, Cao X, Li P, Fan Y, Zhang L, Ma X, Sun R, Liu Y, Li W
Journal of cellular and molecular medicine 2020 Jan;24(1):521-529

---

EGFR-vIII downregulated H2AZK4/7AC though the PI3K/AKT-HDAC2 axis to regulate cell cycle progression.

Zhao H, Wang Y, Yang C, Zhou J, Wang L, Yi K, Li Y, Wang Q, Shi J, Kang C, Zeng L
Clinical and translational medicine 2020 Jan 28;9(1):10

---

Gestational stress induced differential expression of HDAC2 in male rat offspring hippocampus during development.

Liao L, Wang X, Yao X, Zhang B, Zhou L, Huang J
Neuroscience research 2019 Oct;147:9-16

---

SREBP1, targeted by miR-18a-5p, modulates epithelial-mesenchymal transition in breast cancer via forming a co-repressor complex with Snail and HDAC1/2.

Zhang N, Zhang H, Liu Y, Su P, Zhang J, Wang X, Sun M, Chen B, Zhao W, Wang L, Wang H, Moran MS, Haffty BG, Yang Q
Cell death and differentiation 2019 May;26(5):843-859

---

Mucin 1 downregulation impairs the anti-necroptotic effects of glucocorticoids in human bronchial epithelial cells.

Zhang H, Liu Q, Kong L, Xu S
Life sciences 2019 Mar 15;221:168-177

---

Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation.

Zhong S, Li L, Zhang YL, Zhang L, Lu J, Guo S, Liang N, Ge J, Zhu M, Tao Y, Wu YC, Yin H
The Journal of clinical investigation 2019 Jan 2;129(1):252-267

---

LncRNA HOXA-AS2 positively regulates osteogenesis of mesenchymal stem cells through inactivating NF-κB signalling.

Zhu X, Yu J, Du J, Zhong G, Qiao L, Lin J
Journal of cellular and molecular medicine 2019 Feb;23(2):1325-1332

---

Clinical significance of enhancer of zeste homolog 2 and histone deacetylases 1 and 2 expression in peripheral T-cell lymphoma.

Zhang H, Lv H, Jia X, Hu G, Kong L, Zhang T, Li L, Pan Y, Zhai Q, Meng B, Wang X, Wang H, Wang X
Oncology letters 2019 Aug;18(2):1415-1423

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The HDAC Inhibitor Quisinostat (JNJ-26481585) Supresses Hepatocellular Carcinoma alone and Synergistically in Combination with Sorafenib by G0/G1 phase arrest and Apoptosis induction.

He B, Dai L, Zhang X, Chen D, Wu J, Feng X, Zhang Y, Xie H, Zhou L, Wu J, Zheng S
International journal of biological sciences 2018;14(13):1845-1858

---

Disruption of Planar Cell Polarity Pathway Attributable to Valproic Acid-Induced Congenital Heart Disease through Hdac3 Participation in Mice.

Duan HY, Zhou KY, Wang T, Zhang Y, Li YF, Hua YM, Wang C
Chinese medical journal 2018 Sep 5;131(17):2080-2088

---

Hyper-acetylation contributes to the sensitivity of chemo-resistant prostate cancer cells to histone deacetylase inhibitor Trichostatin A.

Xu Q, Liu X, Zhu S, Hu X, Niu H, Zhang X, Zhu D, Nesa EU, Tian K, Yuan H
Journal of cellular and molecular medicine 2018 Mar;22(3):1909-1922

---

Regulatory Roles of Histone Deacetylases 1 and 2 in Pb-induced Neurotoxicity.

Wu Y, Xu Y, Huang X, Ye D, Han M, Wang HL
Toxicological sciences : an official journal of the Society of Toxicology 2018 Apr 1;162(2):688-701

---

HDAC2 was involved in placental P-glycoprotein regulation both in vitro and vivo.

Duan H, Zhou K, Zhang Y, Yue P, Wang T, Li Y, Qiu D, Wu J, Hua Y, Wang C
Placenta 2017 Oct;58:105-114

---

Effect of Histone Deacetylase Inhibition on the Expression of Multidrug Resistance-associated Protein 2 in a Human Placental Trophoblast Cell Line.

Duan HY, Ma D, Zhou KY, Wang T, Zhang Y, Li YF, Wu JL, Hua YM, Wang C
Chinese medical journal 2017 Jun 5;130(11):1352-1360

---

The effect of histone deacetylase inhibition on the expression of P-glycoprotein in human placental trophoblast cell lines.

Duan H, Wang C, Zhou K, Wang T, Li Y, Qiu D, Li Q, Zhang Y, Hua Y
Placenta 2017 Jan;49:37-47

---

Histone deacetylase inhibitor apicidin increases expression of the α-secretase ADAM10 through transcription factor USF1-mediated mechanisms.

Hu XT, Zhu BL, Zhao LG, Wang JW, Liu L, Lai YJ, He L, Deng XJ, Chen GJ
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2017 Apr;31(4):1482-1493

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Capsaicin reactivates hMOF in gastric cancer cells and induces cell growth inhibition.

Wang F, Zhao J, Liu D, Zhao T, Lu Z, Zhu L, Cao L, Yang J, Jin J, Cai Y
Cancer biology & therapy 2016 Nov;17(11):1117-1125

---

USP4 inhibits p53 and NF-κB through deubiquitinating and stabilizing HDAC2.

Li Z, Hao Q, Luo J, Xiong J, Zhang S, Wang T, Bai L, Wang W, Chen M, Wang W, Gu L, Lv K, Chen J
Oncogene 2016 Jun 2;35(22):2902-12

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Expression of hMOF in different ovarian tissues and its effects on ovarian cancer prognosis.

Cai M, Hu Z, Liu J, Gao J, Tan M, Zhang D, Zhu L, Liu S, Hou R, Lin B
Oncology reports 2015 Feb;33(2):685-92

---

BRG1, the ATPase subunit of SWI/SNF chromatin remodeling complex, interacts with HDAC2 to modulate telomerase expression in human cancer cells.

Wu S, Ge Y, Huang L, Liu H, Xue Y, Zhao Y
Cell cycle (Georgetown, Tex.) 2014;13(18):2869-78

---

Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy.

Wang X, Liu J, Zhen J, Zhang C, Wan Q, Liu G, Wei X, Zhang Y, Wang Z, Han H, Xu H, Bao C, Song Z, Zhang X, Li N, Yi F
Kidney international 2014 Oct;86(4):712-25

---

Histone cross-talk connects protein phosphatase 1α (PP1α) and histone deacetylase (HDAC) pathways to regulate the functional transition of bromodomain-containing 4 (BRD4) for inducible gene expression.

Hu X, Lu X, Liu R, Ai N, Cao Z, Li Y, Liu J, Yu B, Liu K, Wang H, Zhou C, Wang Y, Han A, Ding F, Chen R
The Journal of biological chemistry 2014 Aug 15;289(33):23154-23167

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 12922-3-AP(HDAC2 antibody) at dilution of 1:1000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using HDAC2 antibody 12922-3-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-HDAC2 (IP:12922-3-AP, 3ug; Detection:12922-3-AP 1:1000) with mouse testis tissue lysate 10000ug.

Sample type

tissue

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human prostate cancer using 12922-3-AP(HDAC2 antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Flow cytometry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The HDAC2 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human HDAC2. This antibody recognizes human,mouse,rat antigen. The HDAC2 antibody has been validated for the following applications: ELISA, WB, IHC, IF, IP, FC analysis.

Appendix

12922-3-AP.pdf

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