GTX109642

Huang D, Chen J, Yang L, Ouyang Q, Li J, Lao L, Zhao J, Liu J, Lu Y, Xing Y, Chen F, Su F, Yao H, Liu Q, Su S, Song E
Nature immunology 2018 Oct;19(10):1112-1125

Zeb1-Hdac2-eNOS circuitry identifies early cardiovascular precursors in naive mouse embryonic stem cells.

Cencioni C, Spallotta F, Savoia M, Kuenne C, Guenther S, Re A, Wingert S, Rehage M, Sürün D, Siragusa M, Smith JG, Schnütgen F, von Melchner H, Rieger MA, Martelli F, Riccio A, Fleming I, Braun T, Zeiher AM, Farsetti A, Gaetano C
Nature communications 2018 Mar 29;9(1):1281

Pinostrobin Inhibits Proprotein Convertase Subtilisin/Kexin-type 9 (PCSK9) Gene Expression through the Modulation of FoxO3a Protein in HepG2 Cells.

Gao WY, Chen PY, Chen SF, Wu MJ, Chang HY, Yen JH
Journal of agricultural and food chemistry 2018 Jun 20;66(24):6083-6093

Xanthohumol Suppresses Mylip/Idol Gene Expression and Modulates LDLR Abundance and Activity in HepG2 Cells.

Chen SF, Chen PY, Hsu HJ, Wu MJ, Yen JH
Journal of agricultural and food chemistry 2017 Sep 13;65(36):7908-7918

Epigenetic regulation of the glucose transporter gene Slc2a1 by β-hydroxybutyrate underlies preferential glucose supply to the brain of fasted mice.

Tanegashima K, Sato-Miyata Y, Funakoshi M, Nishito Y, Aigaki T, Hara T
Genes to cells : devoted to molecular & cellular mechanisms 2017 Jan;22(1):71-83

Inhibition of HDAC3- and HDAC6-Promoted Survivin Expression Plays an Important Role in SAHA-Induced Autophagy and Viability Reduction in Breast Cancer Cells.

Lee JY, Kuo CW, Tsai SL, Cheng SM, Chen SH, Chan HH, Lin CH, Lin KY, Li CF, Kanwar JR, Leung EY, Cheung CC, Huang WJ, Wang YC, Cheung CH
Frontiers in pharmacology 2016;7:81

Tanshinone IIA Modulates Low Density Lipoprotein Uptake via Down-Regulation of PCSK9 Gene Expression in HepG2 Cells.

Chen HC, Chen PY, Wu MJ, Tai MH, Yen JH
PloS one 2016;11(9):e0162414

A gene expression signature-based approach reveals the mechanisms of action of the Chinese herbal medicine berberine.

Lee KH, Lo HL, Tang WC, Hsiao HH, Yang PM
Scientific reports 2014 Sep 17;4:6394

Down-regulation of the oncogene PTTG1 via the KLF6 tumor suppressor during induction of myeloid differentiation.

Chen PY, Yen JH, Kao RH, Chen JH
PloS one 2013;8(8):e71282

No comments: Submit comment

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: HDAC2 antibody detects HDAC2 protein by western blot analysis.A. 30 ?g C2C12 whole cell lysate/extract7.5% SDS-PAGEHDAC2 antibody (GTX109642) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: HDAC2 antibody detects HDAC2 protein by western blot analysis.A. 30 ?g Rat2 whole cell lysate/extract7.5% SDS-PAGEHDAC2 antibody (GTX109642) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample(30 ug whole cell lysate)A:Hep G2 (GTX27900)7.5% SDS PAGEGTX109642 diluted at 1:1000
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: HDAC2 antibody detects HDAC2 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC2 antibody (GTX109642) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using HDAC2(GTX109642) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: HDAC2 antibody immunoprecipitates HDAC2 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 £gg HeLa whole cell lysate/extract B. Control with 2 £gg of preimmune rabbit IgG C. Immunoprecipitation of HDAC2 protein by 2 £gg of HDAC2 antibody (GTX109642) 7.5% SDS-PAGE The immunoprecipitated HDAC2 protein was detected by HDAC2 antibody (GTX109642) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using HDAC2(GTX109642) antibody at 1:100 dilution.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: HDAC2 antibody immunoprecipitates HDAC2 protein-DNA complex in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 £gg preimmune rabbit IgGB. 5 £gg of HDAC2 antibody (GTX109642)The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter.