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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
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- Product number
- PA5-35395 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-HDAC2 (Ser394) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, chicken, guinea pig, non-human primate, rat and sheep based on 100% sequence homology. This antibody contains enough material to conduct 10 mini-Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- ~0.25 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Phosphoproteome and drug-response effects mediated by the three protein phosphatase 2A inhibitor proteins CIP2A, SET, and PME-1.
Kauko O, Imanishi SY, Kulesskiy E, Yetukuri L, Laajala TD, Sharma M, Pavic K, Aakula A, Rupp C, Jumppanen M, Haapaniemi P, Ruan L, Yadav B, Suni V, Varila T, Corthals GL, Reimand J, Wennerberg K, Aittokallio T, Westermarck J
The Journal of biological chemistry 2020 Mar 27;295(13):4194-4211
The Journal of biological chemistry 2020 Mar 27;295(13):4194-4211
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-HDAC2 pSer394 in mouse heart lysate using a Phospho-HDAC2 pSer394 polyclonal antibody (Product # PA5-35395). Results show a band at ~55kDa. Phosphospecificity is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that the lysate was incubated in lamda-Ptase (800 units/1mg protein for 30 min). The immunolabeling is completely eliminated by treatment with lambda-Ptase.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of HDAC2 in mouse heart lysate showing specific immunolabeling of a band at ~55 kDa corresponding to Phospho-HDAC2 (Ser394) polyclonal antibody (Product # PA5-35395) in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (1,200 units for 30 min).
