PA5-35395

antibody from Invitrogen Antibodies

Targeting: HDAC2 KDAC2, RPD3, YAF1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-35395

Provider

Invitrogen Antibodies

Product name

Phospho-HDAC2 (Ser394) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with bovine, chicken, guinea pig, non-human primate, rat and sheep based on 100% sequence homology.

Concentration

~0.25 mg/mL

References

Submitted references

Phosphoproteome and drug-response effects mediated by the three protein phosphatase 2A inhibitor proteins CIP2A, SET, and PME-1.

Kauko O, Imanishi SY, Kulesskiy E, Yetukuri L, Laajala TD, Sharma M, Pavic K, Aakula A, Rupp C, Jumppanen M, Haapaniemi P, Ruan L, Yadav B, Suni V, Varila T, Corthals GL, Reimand J, Wennerberg K, Aittokallio T, Westermarck J
The Journal of biological chemistry 2020 Mar 27;295(13):4194-4211

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-HDAC2 pSer394 in mouse heart lysate using a Phospho-HDAC2 pSer394 polyclonal antibody (Product # PA5-35395). Results show a band at ~55kDa. Phosphospecificity is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that the lysate was incubated in lamda-Ptase (800 units/1mg protein for 30 min). The immunolabeling is completely eliminated by treatment with lambda-Ptase.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of HDAC2 in mouse heart lysate showing specific immunolabeling of a band at ~55 kDa corresponding to Phospho-HDAC2 (Ser394) polyclonal antibody (Product # PA5-35395) in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (1,200 units for 30 min).