Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 680103 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#680103, RRID:AB_2632895
- Product name
- Go-ChIP-Grade™ Purified anti-HDAC2
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 13G8C67
- Vial size
- 25 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
No comments: Submit comment
Supportive validation
- Submitted by
- BioLegend (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay plate by loading 3 µg of cross-linked chromatin samples from Jurkat cells with either A) 1:100 dilution of Go-ChIP-Grade™ Purified anti-HDAC2 Antibody (clone 13G8C67), B) equal amount of Purified Mouse IgG1, κ Isotype Control Antibody (clone MG1-45), or C) competitor's ChIP-grade Purified anti-HDAC2 Antibody and D) equal amount of matched Isotype Control Antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human FOSL1 (Fos like 1) gene region, which is known to be bound to HDAC2. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.
- Conjugate
- Unconjugated