Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Chromatin Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP2-59269 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Histone Deacetylase 2/HDAC2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 50 ug
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Histone Deacetylase 2/HDAC2 Antibody (C.15200201) [NBP2-59269] - Whole cell extracts (40 ug) from HeLa cells transfected with HDAC2 siRNA (lane 2) and from an untransfected control (lane 1) were analyzed using the antibody against HDAC2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (kDa) is shown on the left.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Histone Deacetylase 2/HDAC2 Antibody (C.15200201) [NBP2-59269] - Whole cell extracts from HeLa cells (25 ug) were analyzed using the antibody against HDAC2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (kDa) is shown on the left.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation: Histone Deacetylase 2/HDAC2 Antibody (C.15200201) [NBP2-59269] - ChIP assays were performed using human HeLa cells, the antibody consisting of 1, 2 and 5 ug per ChIP experiment was analyzed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for the ACTB, EIF4A2 and LMO4 genes, used as positive controls, and for the Sat2 satellite repeat, used as negative control. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
