Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- Ab107031 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant HDAC2 Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human HDAC2 Antibody, Recombinant (R06-4E4), could be used for WB, IHC, IF, ICC and so on.Application:WB: 1/500-1/1000IHC: 1/50-1/100IF/ICC: 1/50-1/200Protein Function:Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 339-488
- Antibody clone number
- R06-4E4
- Vial size
- 100μl,10μl,1ml,50μl
- Concentration
- 0,6 mg/ml
- Storage
- Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant HDAC2 Antibody (Ab107031) - Western Blot All lanes: Recombinant HDAC2 Antibody (Ab107031) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution Predicted band size: 55 kDa Observed band size: 60 kDa Exposure time: 5.0 s
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant HDAC2 Antibody (Ab107031) - Western Blot All lanes: Recombinant HDAC2 Antibody (Ab107031) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution Predicted band size: 55 kDa Observed band size: 60 kDa Exposure time: 29.1 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant HDAC2 Antibody (Ab107031) - IF/ICC Immunocytochemistry analysis of HDAC2 (green) in HeLa using Recombinant HDAC2 Antibody (Ab107031), and cells were counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant HDAC2 Antibody (Ab107031) - IHC Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Recombinant HDAC2 Antibody (Ab107031). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.