Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MAB8125-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Apolipoprotein A-IV/ApoA4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Apolipoprotein A-IV/ApoA4 in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P06727
- Isotype
- IgG
- Antibody clone number
- 988829
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Apolipoprotein A-IV/ApoA4 by Simple WesternTM. Simple Western lane view shows lysates of human plasma, loaded at 0.2 mg/mL. A specific band was detected for Apolipoprotein A-IV/ApoA4 at approximately 51 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Apolipoprotein A-IV/ApoA4 Monoclonal Antibody (Catalog # MAB8125) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Apolipoprotein A-IV/ApoA4 by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Apolipoprotein A-IV/ApoA4 Monoclonal Antibody (Catalog # MAB8125) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Apolipoprotein A-IV/ApoA4 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Apolipoprotein A-IV/ApoA4 in Human Intestine. Apolipoprotein A-IV/ApoA4 was detected in immersion fixed paraffin-embedded sections of human intestine using Mouse Anti-Human Apolipoprotein A-IV/ApoA4 Monoclonal Antibody (Catalog # MAB8125) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.