Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [5]
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Validation data
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- Product number
- NBP1-86179 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-86179, RRID:AB_11008095
- Product name
- Rabbit Polyclonal Apolipoprotein A-IV/ApoA4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Apolipoprotein A-IV/ApoA4 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model.
Global proteomic profiling reveals altered proteomic signature in schizophrenia serum.
Kato BS, Nicholson G, Neiman M, Rantalainen M, Holmes CC, Barrett A, Uhlén M, Nilsson P, Spector TD, Schwenk JM
Proteome science 2011 Nov 17;9:73
Proteome science 2011 Nov 17;9:73
Global proteomic profiling reveals altered proteomic signature in schizophrenia serum.
Levin Y, Wang L, Schwarz E, Koethe D, Leweke FM, Bahn S
Molecular psychiatry 2010 Nov;15(11):1088-100
Molecular psychiatry 2010 Nov;15(11):1088-100
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Apolipoprotein A-IV/ApoA4 Antibody [NBP1-86179] - Analysis in control (vector only transfected HEK293T lysate) and APOA4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody [NBP1-86179] - Analysis in human small intestine and liver tissues. Corresponding APOA4 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody [NBP1-86179] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody [NBP1-86179] - Staining of human colon shows no positivity in glandular cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody [NBP1-86179] - Staining of human liver shows no positivity in hepatocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Apolipoprotein A-IV/ApoA4 Antibody [NBP1-86179] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.