PA1-886

antibody from Invitrogen Antibodies

Targeting: DMAP1 DNMAP1, DNMTAP1, EAF2, FLJ11543, KIAA1425, MEAF2, SWC4

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-886

Provider

Invitrogen Antibodies

Product name

DMAP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1-886 detects DNA methyltransferase 1 associated protein (DMAP1) from human and mouse cells. PA1-886 has been successfully used in Western blot and Immunohistochemistry (paraffin) procedures. By Western blot, this antibody detects an ~52 kDa band which corresponds to DMAP1 from HeLa cell nuclear extracts. PA1-886 immunizing peptide corresponds to amino acid residues 435-450 from mouse DMAP1. This sequence is completely conserved in human DMAP1. PA1-886 immunizing peptide (Cat. # PEP-118) is available for use in neutralization and control experiments.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

The mammalian YL1 protein is a shared subunit of the TRRAP/TIP60 histone acetyltransferase and SRCAP complexes.

Cai Y, Jin J, Florens L, Swanson SK, Kusch T, Li B, Workman JL, Washburn MP, Conaway RC, Conaway JW
The Journal of biological chemistry 2005 Apr 8;280(14):13665-70

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Structural and functional conservation of the NuA4 histone acetyltransferase complex from yeast to humans.

Doyon Y, Selleck W, Lane WS, Tan S, Côté J
Molecular and cellular biology 2004 Mar;24(5):1884-96

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Structural and functional conservation of the NuA4 histone acetyltransferase complex from yeast to humans.

Doyon Y, Selleck W, Lane WS, Tan S, Côté J
Molecular and cellular biology 2004 Mar;24(5):1884-96

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RGS6 interacts with DMAP1 and DNMT1 and inhibits DMAP1 transcriptional repressor activity.

Liu Z, Fisher RA
The Journal of biological chemistry 2004 Apr 2;279(14):14120-8

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Identification of new subunits of the multiprotein mammalian TRRAP/TIP60-containing histone acetyltransferase complex.

Cai Y, Jin J, Tomomori-Sato C, Sato S, Sorokina I, Parmely TJ, Conaway RC, Conaway JW
The Journal of biological chemistry 2003 Oct 31;278(44):42733-6

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DMPA1 was performed by loading 25 µg of HL-60 (Lane 1), Hela (Lane 2), and Mouse brain (Lane 3) cell lysates and a molecular weight protein ladder onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with a blocking buffer at 4ºC overnight. The membrane was probed with a DMAP1 polyclonal antibody (Product # PA1-886) at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Results show a band at 52 kDa in all three cell lysates.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of DMAP1 showing staining in the cytoplasm and nucleus of paraffin-treated human esophageal carcinoma (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a DMAP1 polyclonal antibody (Product # PA1-886) diluted by 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of DMAP1 showing staining in the cytoplasm and nucleus of paraffin-treated mouse brain tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a DMAP1 polyclonal antibody (Product # PA1-886) diluted by 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of DMAP1 showing staining in the cytoplasm and nucleus of paraffin-treated mouse uterus tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a DMAP1 polyclonal antibody (Product # PA1-886) diluted by 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.