Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- BS5797 - Provider product page
- Provider
- Bioworld Technology, Inc
- Product name
- MLF1 polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide, corresponding to amino acids 194-239 of Human MLF1.
- Description
- Myeloid leukemia factor 1 (MLF1) is a 268 amino acid protein expressed by a gene that is involved in translocations associated with acute myeloid leukemia. MLF1 is a widely expressed negative regulator of cell cycle progression functioning upstream of the tumor suppressor p53. MLF1 induces p53-dependent cell cycle arrest in murine embryonic fibroblasts. MLF1 expression also inversely affects the endogenous level of COP1, a ubiquitin ligase for p53, inhibits Epo-induced cell cycle exit, and inhibits a rise in the cell cycle inhibitor p27. Polo-like kinase 1 (Plk1) phosphorylates MLF1 at its Thr78 site, which induces ubiquitination and degradation of MLF1 before the transition from metaphase to anaphase. Mutations of these phosphorylation sites stabilize MLF1 and inhibit mitotic progression. MLF1 normally functions in multi-potent progenitor cells, and its dysregulation may be somewhat responsible for leukemogenesis.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
- Main image
- Experimental details
- Western blot (WB) analysis of MLF1 pAb at 1:500 dilutionLane1:Hela cell lysateLane2:sp2/0 cell lysateLane3:H9C2 cell lysate