Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- OAAB03783 - Provider product page
- Provider
- Aviva Systems Biology
- Product name
- MLF1 antibody - C-terminal region
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 400ul
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- MLF1 Antibody (C-term) western blot analysis in MCF-7 cell lysates (35ug/lane).This demonstrates the MLF1 antibody detected the MLF1 protein (arrow).
- Sample type
- MCF-7 cell line lysates
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of MLF1 Antibody (C-term) in mouse heart tissue lysates (35ug/lane). MLF1 (arrow) was detected using the purified Pab.
- Sample type
- mouse heart tissue lysates
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- MLF1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MLF1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
- Sample type
- testis carcinoma
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- MLF1 Antibody (C-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Sample type
- CEM cells
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol