TA504808

antibody from OriGene

Targeting: MLF1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA504808

Provider

OriGene

Proper citation

OriGene Cat#TA504808, RRID:AB_2622614

Product name

MLF1 (Myeloid leukemia factor 1) mouse monoclonal antibody, clone OTI1G4 (formerly 1G4)

Antibody type

Monoclonal

Description

MLF1 (Myeloid leukemia factor 1) mouse monoclonal antibody, clone OTI1G4 (formerly 1G4)

Host

Mouse

Conjugate

Unconjugated

Epitope

MLF1

Isotype

IgG

Antibody clone number

OTI1G4

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-MLF1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MLF1 (RC202774, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MLF1.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-MLF1 mouse monoclonal antibody (TA504808) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MLF1(RC202774).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC202774 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-MLF1 antibody(TA504808), and then analyzed by flow cytometry.

Validation comment

FC