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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00005889-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005889-M01, RRID:AB_547549
- Product name
- Mouse Monoclonal Rad51C Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. RAD51C - RAD51 homolog C (S. cerevisiae)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rad51C Antibody (3F3-5C6) [H00005889-M01] - Analysis of RAD51C over-expressed 293 cell line, cotransfected with RAD51C Validated Chimera RNAi ( Cat # H00005889-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RAD51C monoclonal antibody (M01), clone 3F3-5C6 (Cat # H00005889-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rad51C Antibody (3F3-5C6) [H00005889-M01] - Analysis of RAD51C expression in transfected 293T cell line by RAD51C monoclonal antibody (M01), clone 3F3-5C6.Lane 1: RAD51C transfected lysate(42.2 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rad51C Antibody (3F3-5C6) [H00005889-M01] - RAD51C monoclonal antibody (M01), clone 3F3-5C6. Analysis of RAD51C expression in 293.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rad51C Antibody (3F3-5C6) [H00005889-M01] - RAD51C monoclonal antibody (M01), clone 3F3-5C6 Analysis of RAD51C expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Rad51C Antibody (3F3-5C6) [H00005889-M01] - Detection limit for recombinant GST tagged RAD51C is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Rad51C Antibody (3F3-5C6) [H00005889-M01] - Analysis of monoclonal antibody to RAD51C on HeLa cell. Antibody concentration 10 ug/ml.
