Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- A301-336A-T - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ERCC3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 10 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human ERCC3 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ERCC3 (Cat. No. A301-336A Lot 2) used at a dilution of 1:10,000 (0.1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human ERRC3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ERRC3 antibody A301-336A (lot A301-336A-2) used for IP at 3 µg per reaction. ERRC3 was also immunoprecipitated by rabbit anti-ERRC3 antibody A301-337A. For blotting immunoprecipitated ERRC3, A301-337A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.