Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoprecipitation [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- NB100-61059 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-61059, RRID:AB_925376
- Product name
- Rabbit Polyclonal XPB Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Immunoprecipitation: XPB Antibody [NB100-61059] - Detection of Human ERCC3 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ERCC3 antibody NB100-61059 used for IP at 3 mcg/mg lysate. ERCC3 was also immunoprecipitated by rabbit anti- ERCC3 antibody NB100-61060, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 10 secon
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: XPB Antibody [NB100-61059] - FFPE section of human bladder cell carcinoma. Affinity purified rabbit anti-ERCC3 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: XPB Antibody [NB100-61059] - FFPE section of human prostate carcinoma. Affinity purified rabbit anti-ERCC3 used at a dilution of 1:500. Detection: DAB staining using Immunohistochemistry Accessory Kit
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: XPB Antibody [NB100-61059] - Human breast carcinoma. Antibody used at a dilution of 1:1,000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: XPB Antibody [NB100-61059] - Detection of human ERCC3 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ERCC3 (NB100-61059). Detection: DAB