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Western blot
Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
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- Product number
- NB100-61060 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-61060, RRID:AB_2098018
- Product name
- Rabbit Polyclonal XPB Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Epstein-Barr virus co-opts TFIIH component XPB to specifically activate essential viral lytic promoters.
G quadruplexes are genomewide targets of transcriptional helicases XPB and XPD.
Verma D, Church TM, Swaminathan S
Proceedings of the National Academy of Sciences of the United States of America 2020 Jun 9;117(23):13044-13055
Proceedings of the National Academy of Sciences of the United States of America 2020 Jun 9;117(23):13044-13055
G quadruplexes are genomewide targets of transcriptional helicases XPB and XPD.
Gray LT, Vallur AC, Eddy J, Maizels N
Nature chemical biology 2014 Apr;10(4):313-8
Nature chemical biology 2014 Apr;10(4):313-8
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: XPB Antibody [NB100-61060] - Detection of Human and Mouse ERCC3 on HeLa whole cell lysate using NB100-61060. ERCC3 was also immunoprecipitated by rabbit anti-ERCC3 antibody NB100-61059.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: XPB Antibody [NB100-61060] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiERCC3 antibody used for WB at 0.1 ug/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: XPB Antibody [NB100-61060] - Detection of human ERCC3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ERCC3 antibody NB100-61060 used for IP at 3 ug per reaction. ERCC3 was also immunoprecipitated by rabbit anti-ERCC3 antibody NB100-61059. For blotting immunoprecipitated ERCC3, NB100-61060 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
