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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- Ab103206 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Ferritin Light Chain Mouse mAb
- Antibody type
- Monoclonal
- Description
- Mouse anti Human Ferritin Light Chain Antibody, Monoclonal (H1), could be used for WB, IHC and so on. ApplicationWB:0.5-2 _g/mLIHC: 5-20 µg/mLProtein FunctionStores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
AA 2-175- Antibody clone number
- H1
- Vial size
- 100_l,10_l,1ml,20_l,50_l
- Concentration
- Lot by Lot
- Storage
- Store at 4_ short term (1-2 weeks). Store at -20_ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Ferritin Light Chain Mouse mAb (Ab103206) - Western Blot All lanes: Ferritin Light Chain Mouse mAb (Ab103206) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution Predicted band size: 20 kDa Observed band size: 20 kDa Exposure time: 224.0 s
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Ferritin Light Chain Mouse mAb (Ab103206) - Western Blot All lanes: Ferritin Light Chain Mouse mAb (Ab103206) at 1/500 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution Predicted band size: 20 kDa Observed band size: 20 kDa Exposure time: 370.7 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Ferritin Light Chain Mouse mAb (Ab103206) - IHC DAB staining on IHC Sample: Human Spleen Tissue Primary Ab: 20 µg/mL Ferritin Light Chain Mouse mAb (Ab103206) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Ferritin Light Chain Mouse mAb (Ab103206) - IHC DAB staining on IHC Sample: Human Liver Tissue Primary Ab: 20 µg/mL Ferritin Light Chain Mouse mAb (Ab103206) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
