PA5-27357

antibody from Invitrogen Antibodies

Targeting: FTL MGC71996, NBIA3

Antibody data

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Validation data

Product number: PA5-27357 - Provider product page
Provider: Invitrogen Antibodies
Product name: Ferritin Light Chain Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: HepG2, mouse liver. Predicted reactivity: Dog (86%), Cat (85%), Pig (87%), Rabbit (86%), Rhesus Monkey (96%), Bovine (83%), Guinea pig (85%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.268 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

FTL protein structure - PA5-27357 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of FTL using 30 µg of NIH-3T3 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a FTL polyclonal antibody (Product # PA5-27357) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Ferritin Light Chain was performed by separating 50 µg of mouse tissue extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a Ferritin Light Chain Polyclonal Antibody (Product # PA5-27357) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using Ferritin Light Chain Polyclonal Antibody (Product # PA5-27357). Sample (30 µg of whole cell lysate). A: Hep G2. 12% SDS PAGE. Ferritin Light Chain Polyclonal Antibody (Product # PA5-27357) diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Ferritin Light Chain Polyclonal Antibody (Product # PA5-27357) and a 20kDa band corresponding to Ferritin Light Chain was observed across tissues tested with lower levels in mouse spleen. Tissue extracts (30 µg lysate) of Mouse Liver (Lane 1), Rat Liver (Lane 2), Mouse Spleen (Lane 3) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Ferritin is a holoenzyme shell with a molecular weight of about 450 kDa, and consists of 24 subunits of two types, H(heavy) and L(light), hence band above 260kDa could correspond to the holoenzyme shell of 450kDa.