Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-28660 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GLYCTK Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, HepG2. Predicted reactivity: Bovine (80%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.54 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GLYCTK Polyclonal Antibody (Product # PA5-28660). Sample (30 µg of whole cell lysate). A: A431. B: Hep G2. 7.5% SDS PAGE. GLYCTK Polyclonal Antibody (Product # PA5-28660) diluted at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of GLYCTK showing staining in the cytoplasm of HepG2 cells. HepG2 cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min and stained using a GLYCTK polyclonal antibody (Product # PA5-28660) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GLYCTK Polyclonal Antibody detects GLYCTK protein at cytoplasm by confocal immunofluorescent analysis. Sample: HepG2 cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min. Green: GLYCTK protein stained by GLYCTK Polyclonal Antibody (Product # PA5-28660) diluted at 1:500. Blue: Hoechst 33342 staining. [Images captured by Olympus FV10i Confocal Laser Scanning Microscope].
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using GLYCTK (Product # PA5-28660) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.