LS-C788803

antibody from LSBio

Targeting: PRR11 FLJ11029

Western blot (Enhanced validation)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

LS-C788803

Provider

LSBio

Product name

PRR11 Antibody (aa125-360, clone OTI2A10, Carrier-free) LS-C788803

Antibody type

Monoclonal

Description

Purified from ascites.

Reactivity

Human, Simian

Host

Mouse

Isotype

IgG

Antibody clone number

OTI2A10

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRR11 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRR11.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot of extracts (35ug) from 9 different cell lines by using anti-PRR11 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PRR11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded Human breast tissue using anti-PRR11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Human breast tissue using anti-PRR11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PRR11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).