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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [3]
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- Product number
- PA5-36851 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-HSF1 (Ser303) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 82 kDa. Purity is >95% by SDS-PAGE.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-HSF1 pSer303 using Phospho-HSF1 pSer303 polyclonal antibody (Product # PA5-36851) at a dilution of 1:500. Lane 1: Hela cell lysate treated with TNF-alpha (20 ng/mL, 15 min), Lane 2: Raw264.7 cell lysate treated with TNF-alpha (20 ng/mL, 15 min), Lane 3: PC12 cell lysate treated with TNF-alpha (20 ng/mL, 15 min).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Phospho-HSF1 (Ser303) in paraffin-embedded human breast carcinoma tissue (cytoplasmic and nucleus staining) and negative control (right, with PBS only). Samples were incubated with Phospho-HSF1 (Ser303) polyclonal antibody (Product # PA5-36851) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-HSF1 pSer303 in paraffin-embedded human brain tissue using Phospho-HSF1 pSer303 polyclonal antibody (Product # PA5-36851).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-HSF1 pSer303 in paraffin-embedded human brain tissue using Phospho-HSF1 pSer303 polyclonal antibody (Product # PA5-36851).
