Antibody data
- Antibody Data
 - Antigen structure
 - References [0]
 - Comments [0]
 - Validations
 - Western blot [6]
 - Immunocytochemistry [1]
 - Immunohistochemistry [4]
 - Other assay [1]
 
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Validation data
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- Product number
 - 51034-1-AP - Provider product page

 - Provider
 - Invitrogen Antibodies
 - Product name
 - HSF1 Polyclonal Antibody
 - Antibody type
 - Polyclonal
 - Antigen
 - Other
 - Reactivity
 - Human, Mouse, Rat
 - Host
 - Rabbit
 - Isotype
 - IgG
 - Vial size
 - 150 µL
 - Concentration
 - 0.52 mg/mL
 - Storage
 - -20°C
 
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					Supportive validation
					
									
				
				- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - K-562 cells were subjected to SDS PAGE followed by western blot with 51034-1-AP (HSF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
 
- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - HEK-293 cells were subjected to SDS PAGE followed by western blot with 51034-1-AP (HSF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
 
- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - Jurkat cells were subjected to SDS PAGE followed by western blot with 51034-1-AP (HSF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
 
- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - A431 cells were subjected to SDS PAGE followed by western blot with 51034-1-AP (HSF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
 
- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - Mouse heart tissue were subjected to SDS PAGE followed by western blot with 51034-1-AP (HSF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
 
- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - HeLa cells were subjected to SDS PAGE followed by western blot with 51034-1-AP (HSF1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.
 
							
					Supportive validation
					
									
				
				- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - Immunofluorescent analysis of A431 cells using 51034-1-AP (HSF1 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IGG.
 
							
					Supportive validation
					
									
				
				- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 51034-1-AP (HSF1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
 
- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - Immunohistochemistry of paraffin-embedded human testis tissue slide using 51034-1-AP (HSF1 Antibody) at dilution of 1:50 (under 10x lens).
 
- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - Immunohistochemistry of paraffin-embedded human testis tissue slide using 51034-1-AP (HSF1 Antibody) at dilution of 1:50 (under 40x lens).
 
- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 51034-1-AP ( HSF1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
 
							
					Supportive validation
					
									
				
		- Submitted by
 - Invitrogen Antibodies (provider)
 - Main image
 
- Experimental details
 - IP result of anti-HSF1 (IP:51034-1-AP, 4ug; Detection:51034-1-AP 1:1000) with Jurkat cells lysate 2400ug.