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Western blot
Immunoprecipitation
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- A303-175A-T - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HSF1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 10 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human HSF1 by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- HSF1 Cat. No. A303-174A Lot1 used at a dilution of 1:5,000 (0.2µg/ml) (left), Cat. No. A303-175A Lot1 used at a dilution of 1:5,000 (0.2µg/ml) (center) and Cat. No. A303-176A Lot1 used at a dilution of 1:1,000 (0.2µg/ml) (right). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human HSF1 by immunohistochemistry. Samples: FFPE sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- HSF1 Cat. No. A303-174A Lot1 used at a dilution of 1:1,000 (1µg/ml) (left), Cat. No. A303-175A Lot1 used at a dilution of 1:1,000 (1µg/ml) (center) and Cat. No. A303-176A Lot1 used at a dilution of 1:200 (1µg/ml) (right). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human HSF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HSF1 antibody A303-175A used for IP at 6 µg/mg lysate. HSF1 was also immunoprecipitated by rabbit anti-HSF1 antibodies A303-174A and A303-176A, which recognize other epitopes. For blotting immunoprecipitated HSF1, A303-176A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
