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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- OAAB06452 - Provider product page
- Provider
- Aviva Systems Biology
- Product name
- GPR17 antibody - center region
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 400ul
- Storage
- Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of GPR17 Antibody (Center) in MDA-MB435 cell lysates (35ug/lane). GPR17 (arrow) was detected using the purified Pab.
- Sample type
- MDA-MB435 cell line lysates
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- GPR17 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GPR17 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
- Sample type
- brain tissue
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- GPR17 Antibody (Center) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Sample type
- MDA-MB435 cells
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
