MA5-27501

antibody from Invitrogen Antibodies

Targeting: GPR17

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-27501

Provider

Invitrogen Antibodies

Product name

GPR17 Monoclonal Antibody (OTI17C1)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

OTI17C1

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded human adult brain tissue. To expose target proteins, heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min. Following antigen retrieval, tissues were probed with a GPR17 monoclonal antibody (Product # MA5-27501) at a dilution of 1:600.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded mouse cerebrum tissue. To expose target proteins, heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min. Following antigen retrieval, tissues were probed with a GPR17 monoclonal antibody (Product # MA5-27501) at a dilution of 1:150.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded mouse cerebrum tissue. To expose target proteins, heat-induced epitope retrieval by Tris-EDTA buffer (pH8.0) at 120°C for 2.5 min. Following antigen retrieval, tissues were probed with a GPR17 monoclonal antibody (Product # MA5-27501) at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded human embryonic cerebellum tissue. To expose target proteins, heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min. Following antigen retrieval, tissues were probed with a GPR17 monoclonal antibody (Product # MA5-27501) at a dilution of 1:600.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded rat cerebrum tissue. To expose target proteins, heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min. Following antigen retrieval, tissues were probed with a GPR17 monoclonal antibody (Product # MA5-27501) at a dilution of 1:150.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded rat cerebrum tissue. To expose target proteins, heat-induced epitope retrieval by Tris-EDTA buffer (pH8.0) at 120°C for 2.5 min. Following antigen retrieval, tissues were probed with a GPR17 monoclonal antibody (Product # MA5-27501) at a dilution of 1:200.