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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [2]
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- Product number
- NB100-374 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-374, RRID:AB_10002713
- Product name
- Rabbit Polyclonal SMC4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references RBF1 promotes chromatin condensation through a conserved interaction with the Condensin II protein dCAP-D3.
Longworth MS, Herr A, Ji JY, Dyson NJ
Genes & development 2008 Apr 15;22(8):1011-24
Genes & development 2008 Apr 15;22(8):1011-24
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMC4 Antibody [NB100-374] - Detection of Human SMC4 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMC4 antibody NB100-374 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMC4 Antibody [NB100-374] - Whole cell lysate (90 ug) from 293T cells (lane 1), SMC4 transfected 239T cells (lane 2) or normal human lymphoblasts (lane 3) separated on an 8% SDS-PAGE gel. Antibody used at 0.2 ug/ml and goat anti-rabbit HRP used at a dilution of 1:20,000.
