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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- MA5-57722 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMC4 Recombinant Rabbit Monoclonal Antibody (24GB2400)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 24GB2400
- Vial size
- 100 µL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SMC4. SMC4 expression in wild type (WT) and SMC4 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 alpha serves as a loading control. The blot was incubated with SMC4 Recombinant Monoclonal Antibody (Product # MA5-57722) at a 1:5,000 dilution and HRP-conjugated goat anti-rabbit secondary antibody at a 1:20,000 dilution respectively.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SMC4. Total cell lysates (30 μg) from C2C12, H9c2, HAP-1, HeLa, HepG2, and HT-1080 cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SMC4 Recombinant Monoclonal Antibody (Product # MA5-57722) at a 1:5,000 dilution and HRP-conjugated goat anti-rabbit secondary antibody at a 1:20,000 dilution respectively.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical staining of SMC4 in HT-1080 cells using SMC4 Recombinant Monoclonal Antibody (Product # MA5-57722) at a 1:1,1000 dilution. Nuclei were stained blue with DAPI; SMC4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of SMC4 expression in HT-1080 cells using SMC4 Recombinant Monoclonal Antibody (Product # MA5-57722) at a 1:2,000 dilution. Green, isotype control; red, SMC4.
