Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- A010-21AP - Provider product page
- Provider
- Badrilla Ltd
- Product name
- Anti-SERCA Y/IF4 (AP) Mouse monoclonal IgG
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- SERCA1 is the Ca2+-pump (109kDa) located in the sarcoplasmic reticulum (SR) of fast-twitch skeletal muscle and SERCA2 is the Ca2+-pump located in the sarcoplasmic reticulum (SR) of cardiac, smooth and slow-twitch skeletal muscle. The enzyme transports 2 Ca2+ from the cytoplasm into the SR using the hydrolysis of 1 molecule of ATP to power the reaction. This terminates muscle contraction and promotes muscle relaxation. Mutations in SERCA1 are associated with Brody disease; mutations in SERCA2 are associated with Darier disease and down regulation of SERCA2 is seen in heart failure. The monoclonal antibody Y/1F4 was produced by immunizing mice with purified SERCA1: the antibody inhibits SERCA1 activity upon binding (Colyer et al., 1989). Antibody Y/1F4 recognises both SERCA1 and SERCA2.
- Reactivity
- Chicken/Avian
- Host
- Mouse
- Antigen sequence
VGNKMFVKGA
- Isotype
- IgG
- Vial size
- 50 µl
- Storage
- Lyophilised: 4 °C Reconstituted:-80 °C
- Handling
- Reconstitute lyophilised powder in 50 µl of 18 MΩ H2O, aliquot and store frozen at -80 °C for 1 year. Avoid freeze - thaw cycles
Submitted references Effects on ATPase activity of monoclonal antibodies raised against (Ca2+ + Mg2+)-ATPase from rabbit skeletal muscle sarcoplasmic reticulum and their correlation with epitope location.
Colyer J, Mata AM, Lee AG, East JM
The Biochemical journal 1989 Sep 1;262(2):439-47
The Biochemical journal 1989 Sep 1;262(2):439-47
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Supportive validation
- Submitted by
- Badrilla Ltd (provider)
- Main image
- Experimental details
- Western blot
- Sample type
- Homogenates of rat brain, heart and muscle and canine sarcoplasmic reticulum
- Validation comment
- Ab stains for both muscle isoform SERCA1 and cardiac isoform SERCA2
- Primary Ab dilution
- 1.430555556
- Secondary Ab dilution
- 1.430555556
- Protocol
- Protocol