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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- R33420 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- SERCA2 Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific SERCA2 antibody is suitable for use in Western blot/Immunofluorescence/Immunocytochemistry/Immunohistochemistry/ELISA applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
- Storage
- Aliquot and store the SERCA2 antibody at -20oC.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human [A], mouse [B] and rat [C] heart lysates with SERCA2 antibody at 0.1ug/ml. Predicted molecular weight: ~110kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human [A], mouse [B] and rat [C] heart lysates with SERCA2 antibody at 0.1ug/ml. Predicted molecular weight: ~110kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IF/ICC staining of fixed and permeabilized human HeLa cells with SERCA2 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human heart tissue with SERCA2 antibody at 5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
