H00007984-M01

antibody from Abnova Corporation

Targeting: ARHGEF5 GEF5, P60, TIM, TIM1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00007984-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00007984-M01, RRID:AB_425754

Product name

ARHGEF5 monoclonal antibody (M01), clone 3A12-B5

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant ARHGEF5.

Antigen sequence

MGGFSRRCSKLINSSQLLYQEYSDVVLNKEIQSQQ
RLESLSETPGPSSPRQPRKALVSSESYLQRLSMAS
SGSLWQEIPVVRNSTVLLSMTHEDQKLQEVKFELI
VSEASYLRSLNIAVDHFQLSTSLRATLSNQEHQWL
FSRLQDVRDVSATFLSDLEENFENNIFSFQVCDVV
LNHAPDFRRVYLPYVTNQTYQERTFQSLMNSNSNF
REVLEKLESDPVCQRLSLKSFLILPFQRITRLKLL
LQNILKRTQPGSSEEAEATKAHHALEQLIRDCNNN
VQSMRRTEELIYLSQKIEFECKIFPLISQSRWLVK
SGELTALEFSASPGLRRKLNTRPVHLHLFNDCLLL
SRPREGSRFLVFDHAPFSSIRGEKCEMKLHGPHKN
LFRLFLRQNTQGAQAEFLFRTETQSEKLRWISALA
MPREELDLLECYNSPQVQCLRAYKPRENDELALEK
ADVVMVTQQSSDGWLEGVRLSDGERGWFPVQQVEF
ISNPEVRAQNLKEAHRVKTAKLQLVEQQA


Isotype

IgG

Antibody clone number

3A12-B5

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Identification of gains on 1q and epidermal growth factor receptor overexpression as independent prognostic markers in intracranial ependymoma.

Mendrzyk F, Korshunov A, Benner A, Toedt G, Pfister S, Radlwimmer B, Lichter P
Clinical cancer research : an official journal of the American Association for Cancer Research 2006 Apr 1;12(7 Pt 1):2070-9

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of ARHGEF5 expression in transfected 293T cell line by ARHGEF5 monoclonal antibody (M01), clone 3A12-B5.Lane 1: ARHGEF5 transfected lysate(60.1 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged ARHGEF5 is 1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol