Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00007984-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00007984-M02, RRID:AB_2060053
- Product name
- Mouse Monoclonal ARHGEF5 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ARHGEF5 Antibody (4D10-D11) [H00007984-M02] - Analysis of ARHGEF5 expression in transfected 293T cell line by ARHGEF5 monoclonal antibody (M02), clone 4D10-D11.Lane 1: ARHGEF5 transfected lysate (Predicted MW: 60.1 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: ARHGEF5 Antibody (4D10-D11) [H00007984-M02] - Detection limit for recombinant GST tagged ARHGEF5 is 3 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: ARHGEF5 Antibody (4D10-D11) [H00007984-M02] - Analysis of ARHGEF5 transfected lysate using anti-ARHGEF5 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ARHGEF5 MaxPab rabbit polyclonal antibody.