Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- MAB8096 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Patched 2/PTCH2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse Patched 2/PTCH2 in ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse Patched 1 or recombinant human Patched 2 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
O35595
- Isotype
- IgG
- Antibody clone number
- 865619
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Patched 2/PTCH2 in Mouse Testis. Patched 2/PTCH2 was detected in immersion fixed frozen sections of mouse testis using Rat Anti-Mouse Patched 2/PTCH2 Monoclonal Antibody (Catalog # MAB8096) at 5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.