PA1-46223

antibody from Invitrogen Antibodies

Targeting: PTCH2

Provider product page for PA1-46223

Western blot

ELISA

Immunohistochemistry

Other assay

Antibody data

Antibody Data
Antigen structure
References [2]
Comments [0]
Validations
Other assay [3]

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Product number: PA1-46223 - Provider product page
Provider: Invitrogen Antibodies
Product name: PTCH2 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Other
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

PTCH2 protein structure - PA1-46223 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig 5 Ptch1/2 and co-receptors are differentially expressed in PGCs and somatic cells. a Expression of Ptch1, Ptch2, Gas1, Boc and Cdon demonstrated by immunofluorescence analyses of primary cultures of dissected E10.5 mouse GR, with co-staining of SSEA1. Representative images from three independent experiments are shown. Scale bar, 50 mum. b Single-cell suspensions from freshly-dissected GR of E10.5 GFP Stella embryos were sorted by FACS (Supplementary Fig. 4 ) and the expression of Ptch1, Ptch2, GFP and Stella in GFP-positive (PGCs) and GFP-negative (somatic cells) populations analysed by quantitative RT-PCR. Each data point represents one experiment. Values normalised to 18s rRNA were plotted with error bars representing SD ( n = 3) after unpaired t -test (* P < 0.05, ** P < 0.005, *** P < 0.001). c Immunofluorescence analyses of Gas1, Ptch2, p-Src, Boc and SSEA1 on the GR tissue sections of WT and Gas1 KO embryos at E10.5. The merged images are shown without DAPI signal for improved clarity. Representative images from three independent experiments are shown. Scale bar, 10 um. d Dot plots showing the relative fluorescence intensity values of Gas1, Ptch2, p-Src, Boc signal observed in PGCs which were normalised with the fluorescence intensity values of SSEA in each cell. Gas1 KO embryos showed a significant decrease in signal intensity in Gas1 (WT ( n = 12), KO ( n = 5), **** P < 0.0001) and p-Src (WT ( n = 7), KO ( n = 12), ** P = 0.0054) but no significant changes in Ptch

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 6 Gas1 mediates Hh ligand reception by Ptch2. a Representative images of at least two independent immunoblotting experiments in which NIH3T3/Cas9 cells with Ptch1 KO were transfected with Myc-tagged Ptch2 expression construct and stimulated with Shh protein in serum-free medium for 40 min. Interactions of Ptch2 with endogenous Smo, Gas1 and Boc were analysed by immunoprecipitation (IP) and Western blotting (WB). IP was performed using specific antibodies as indicated and analysed by WB with respective antibodies as shown. The images in the bottom panel are from Ptch1 KO cells transfected with empty vector, which did not show any co-precipitation of endogenous Gas1 and Ptch2 regardless of Shh treatment, confirming the specificity of these antibodies (bottom panel). b A schematic diagram illustrating the protein interactions before and after Shh treatment. c NIH3T3/Cas9 cells with Gas1 KO were transfected with Myc-tagged Ptch2 and stimulated with Shh protein in serum-free medium for 40 min. IP was performed with anti-Myc antibody. Representative blots from two independent experiments are shown. d A schematic diagram illustrating the protein interactions in Gas1 KO cells. Source data are provided as a Source Data file.