Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [5]
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- Product number
- PA5-38726 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-TRIM59 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Cancer-derived exosomal TRIM59 regulates macrophage NLRP3 inflammasome activation to promote lung cancer progression.
An TRIM59-CDK6 axis regulates growth and metastasis of lung cancer.
Liang M, Chen X, Wang L, Qin L, Wang H, Sun Z, Zhao W, Geng B
Journal of experimental & clinical cancer research : CR 2020 Aug 31;39(1):176
Journal of experimental & clinical cancer research : CR 2020 Aug 31;39(1):176
An TRIM59-CDK6 axis regulates growth and metastasis of lung cancer.
Geng B, Liang M, Qin L, Zhao W, Wang H, Wang L, Pan X, Chen X
Journal of cellular and molecular medicine 2019 Feb;23(2):1458-1469
Journal of cellular and molecular medicine 2019 Feb;23(2):1458-1469
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TRIM59/TSBF-1 in extracts from NIH-3T3 cells using a TRIM59/TSBF-1 polyclonal antibody (Product # PA5-38726).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Tripartite motif-containing protein 59 was performed using 70% confluent log phase THP-1 cells differentiated to M0 macrophages treated with 1 µg/mL of Lipopolysaccharide for 24h. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TRIM59 Polyclonal Antibody (Product # PA5-38726) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2500), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing endoplasmic reticulum localization. Panel e represents untreated cells shows higher expression of TRIM59. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60x magnification.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TRIM59/TSBF-1 in HeLa cells using a TRIM59/TSBF-1 polyclonal antibody (Product # PA5-38726).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TRIM59/TSBF-1 in paraffin-embedded human brain tissue using a TRIM59/TSBF-1 polyclonal antibody (Product # PA5-38726).
Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Roles of TRIM 59 in promoting lung cancer growth and metastasis. (A) Expression levels of TRIM 59 in the indicated lung cancer cell lines were analysed by western blotting (upper). Confirmation of TRIM 59 knockdown in A549 and H1299 cells (lower). (B) CCK 8 assays were used to analyse proliferation of cells stably transfected with Scrambled sh RNA (shCtrl) or TRIM 59 sh RNA (sh TRIM 59). (C) EdU incorporation assay was used to analyse DNA synthesis rates of each transfected cell population. Scale bars, 100 mum. (D, E) Invasion of lung cells stably infected with shCtrl or sh TRIM 59 was assessed by the transwell invasion assays (scale bars, 100 mum) and wound-healing assays (scale bars, 200 mum). Error bars indicate means +- SEM . All these experiments have been repeated three times. The significance was determined by Student t test. * P < 0.05; ** P < 0.01
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 1 Exosomal TRIM59 is characteristically secreted by lung cancer cells and transferred to and internalized by macrophages via exosomes. a . Exosomes released by H1299 and A549 cells were detected by electron microscopy. Scale bar, 100 nm. Immunoblotting assay of indicated proteins in exosomes from H1299 and A549. b . Western blot evaluations were used to evaluateTRIM59 expression in both LC cells (H1299 and A549) exosomes and LC cells treated with TRIM59 shRNA or scrambled controls. c - d . Representative immunofluorescence image shows the internalization of PKH67-labeled H1299-derived exosomes (green) by macrophages. Confocal imaging showed the delivery of PKH67-labeled exosomes (green) to macrophages. Green dots represented delivered exosomes. Scale bar, 150 mum. e - f . THP-1 macrophages cells were incubated with exosomes for the noted periods of time or the noted doses. Western blot evaluations were used to evaluate TRIM59
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- Invitrogen Antibodies (provider)
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- Experimental details
- Fig. 7 TRIM59 stimulates macrophages to facilitate tumor growth and metastasis in vivo. a . Representative image of tumor growth in WT and Tg TRIM59 mice subcutaneously inoculated with LLC cells ( n = 5 per group). b . The growth curve of subcutaneous tumor in WT and Tg TRIM59 mice. And comparison of tumor weight from two groups at the end of the experiment. c . LLC cells (5.0 x 10 6 /100 mul of DMEM) were intravenously injected into WT and Tg TRIM59 mice via the tail vein ( n = 6 in each group). Mice were sacrificed for further observation on day 14. Representative images of lung metastasis in WT and Tg TRIM59 mice. The total area of invasive lesions on the lung slice section represents the invasive tumor volume in the lungs. Immunoblotting assay of TRIM59 in TAMs from the lung metastases of WT or Tg TRIM59 mice. Scale bars, 200 mum. d . Fluorescent multiplex immunohistochemistry (mIHC) staining of adjacent noncancerous tissues and LC tissues. Representative image of an LC case was shown with TRIM59 and IL-1beta co-expression (DAPI, blue; TRIM59, red; IL-1beta, green; CD68, yellow). Scale bars, 200 mum. e . multiplex immunohistochemistry analysis of TRIM59 and IL-1beta protein levels in lung cancer samples on tissue microarrays. The expression of TRIM59 and IL-1beta expression in TAMs were measured with mean fluorescence intensities (MFIs) (in arbitrary units, a.u. ), respectively. The Pearson correlation between TRIM59 and IL-1beta expression ( n = 90; p < 0.01, r = 0.414).
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- Experimental details
- Additional file 1: Figure S1. Exosomal TRIM59 is characteristically secreted by lung cancer cells and transferred to and internalized by macrophages via exosomes. A-B. Representative immunofluorescence image showed the internalization of PKH67-labeled A549-derived exosomes (green) by macrophages. Confocal imaging showed the delivery of PKH67-labeled exosomes (green) to macrophages. Green dots represented delivered exosomes. Scale bar, 150 mum. C-D. Green fluorescent protein (GFP)-tagged TRIM59 was expressed in H1299 and A549 cells and the LC cells-exosomes were isolated and incubated with THP-1 macrophages. The GFP-tagged TRIM59 was detected in THP-1 macrophages. Scale bar, 150 mum. E-F. THP-1 macrophages cells were incubated with exosomes from A549 for the noted periods of time or the noted doses. Western blot evaluations were used to evaluate TRIM59.
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Additional file 2: Figure S2. Overexpression of TRIM59 in macrophages potentiates LC growth and metastasis in mice. A. TRIM59 mRNA levels increased in peritoneal macrophages (PMs) from Tg TRIM59 mice versus WT mice. LLC cells were subcutaneously injected to C57BL/6 WT and transgenic mice. Sixteen days later, PMs of tumor-free mice (NPM) or LLC tumor-bearing mice (TPM) were collected for mRNA assay with real-time PCR. Western blot evaluations were used to evaluate TRIM59. Histograms show means+-s.e.m., ** p < 0.01 (Student's t- test). B. WT and Tg TRIM59 mice subcutaneously inoculated with LLC cells. All mice were euthanized at indicated time. Lung, liver, and kidney tissues of the indicated mice were harvested. Representative images of H&E stained lung, liver, and kidney sections are shown. In both groups of mice, no metastatic lesions was found in the lungs, liver and kidneys. Scale bar: 200 mum. C. TRIM59 mRNA levels increased in TAMs in the lung metastases from Tg TRIM59 mice versus WT mice. Histograms show means+-s.e.m., ** p < 0.01 (Student's t- test).