PA5-21749

antibody from Invitrogen Antibodies

Targeting: KPNA4 IPOA3, MGC12217, MGC26703, QIP1, SRP3

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21749

Provider

Invitrogen Antibodies

Product name

KPNA4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: 293T, HeLa, HepG2, Molt-4, Raji, A549. Predicted reactivity: Chimpanzee (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.04 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Ginsenoside Rg3 inhibits osteosarcoma progression by reducing circ_0003074 expression in a miR-516b-5p/KPNA4-dependent manner.

Wang T, Zhang C, Wang S
Journal of orthopaedic surgery and research 2021 Dec 20;16(1):724

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The Extended C-Terminal Region of Influenza C Virus Nucleoprotein Is Important for Nuclear Import and Ribonucleoprotein Activity.

Tang YS, Lo CY, Mok CK, Chan PK, Shaw PC
Journal of virology 2019 May 1;93(9)

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of QIP1 in paraformaldehyde-fixed HeLa cells using a QIP1 polyclonal antibody (Product # PA5-21749) at a 1:200 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using QIP1 (Product # PA5-21749) antibody at 1:200 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using QIP1 (Product # PA5-21749) antibody at 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using KPNA4 (Product # PA5-21749) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 6 Circ_0003074 regulated KPNA4 expression through miR-516b-5p. A The schematic illustration showing the complementary sites of miR-516b-5p with KPNA4. B , C Dual-luciferase reporter assay was employed to identify the interaction between miR-516b-5p and KPNA4. D , E KPNA4 protein expression was detected by Western blotting in both MG63 cells and Saos2 cells treated with Rg3 at a dose of 0, 40, 80 or 120 uM. F - H KPNA4 expression was detected by qRT-PCR and Western blotting in hFOB1.19 cells, MG63 cells, Saos2 cells, OS tissues and adjacent normal tissues. I , J Spearman correlation analysis was used to confirm the linear correlation between KPNA4 and miR-516b-5p or circ_0003074 in expression in OS tissues. K , L The efficiency of both miR-516b-5p and KPNA4 knockdown was checked by qRT-PCR or Western blotting in MG63 cells and Saos2 cells. M , N The effects between miR-516b-5p knockdown and KPNA4 silencing on KPNA4 expression were determined by Western blotting. O , P Western blotting was carried out to investigate the effects between circ_0003074 overexpression and miR-516b-5p upregulation on KPNA4 protein production. * P < 0.05, ** P < 0.01 and *** P < 0.001