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LearnPA5-21749
antibody from Invitrogen Antibodies
Targeting: KPNA4
IPOA3, MGC12217, MGC26703, QIP1, SRP3
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-21749 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KPNA4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: 293T, HeLa, HepG2, Molt-4, Raji, A549. Predicted reactivity: Chimpanzee (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.04 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Ginsenoside Rg3 inhibits osteosarcoma progression by reducing circ_0003074 expression in a miR-516b-5p/KPNA4-dependent manner.
The Extended C-Terminal Region of Influenza C Virus Nucleoprotein Is Important for Nuclear Import and Ribonucleoprotein Activity.
Wang T, Zhang C, Wang S
Journal of orthopaedic surgery and research 2021 Dec 20;16(1):724
Journal of orthopaedic surgery and research 2021 Dec 20;16(1):724
The Extended C-Terminal Region of Influenza C Virus Nucleoprotein Is Important for Nuclear Import and Ribonucleoprotein Activity.
Tang YS, Lo CY, Mok CK, Chan PK, Shaw PC
Journal of virology 2019 May 1;93(9)
Journal of virology 2019 May 1;93(9)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of KPNA4 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS PAGE. Proteins were transferred to a membrane and probed with a KPNA4 Polyclonal Antibody (Product # PA5-21749) at a dilution of 1:1000. A: Hela, B: Hep G2.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of KPNA4 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS PAGE. Proteins were transferred to a membrane and probed with a KPNA4 Polyclonal Antibody (Product # PA5-21749) at a dilution of 1:1000. A: Hela, B: Hep G2.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using KPNA4 Polyclonal Antibody (Product # PA5-21749). Sample (30 µg of whole cell lysate). Lane A: 293T. Lane B: HepG2. Lane C: Molt-4 . 7.5% SDS PAGE. KPNA4 Polyclonal Antibody (Product # PA5-21749) diluted at 1:1500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- KPNA4 Polyclonal Antibody detects KPNA4 protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract. B. 30 µg GL261 whole cell lysate/extract. C. 30 µg C8D30 whole cell lysate/extract. D. 30 µg NIH-3T3 whole cell lysate/extract. E. 30 µg BCL-1 whole cell lysate/extract.7.5 % SDS-PAGE. KPNA4 Polyclonal Antibody (Product # PA5-21749) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- KPNA4 Polyclonal Antibody detects KPNA4 protein by Western blot analysis. A. 30 µg Rat2 whole cell lysate/extract.7.5 % SDS-PAGE. KPNA4 Polyclonal Antibody (Product # PA5-21749) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of QIP1 in paraformaldehyde-fixed HeLa cells using a QIP1 polyclonal antibody (Product # PA5-21749) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using QIP1 (Product # PA5-21749) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using KPNA4 (Product # PA5-21749) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 6 Circ_0003074 regulated KPNA4 expression through miR-516b-5p. A The schematic illustration showing the complementary sites of miR-516b-5p with KPNA4. B , C Dual-luciferase reporter assay was employed to identify the interaction between miR-516b-5p and KPNA4. D , E KPNA4 protein expression was detected by Western blotting in both MG63 cells and Saos2 cells treated with Rg3 at a dose of 0, 40, 80 or 120 uM. F - H KPNA4 expression was detected by qRT-PCR and Western blotting in hFOB1.19 cells, MG63 cells, Saos2 cells, OS tissues and adjacent normal tissues. I , J Spearman correlation analysis was used to confirm the linear correlation between KPNA4 and miR-516b-5p or circ_0003074 in expression in OS tissues. K , L The efficiency of both miR-516b-5p and KPNA4 knockdown was checked by qRT-PCR or Western blotting in MG63 cells and Saos2 cells. M , N The effects between miR-516b-5p knockdown and KPNA4 silencing on KPNA4 expression were determined by Western blotting. O , P Western blotting was carried out to investigate the effects between circ_0003074 overexpression and miR-516b-5p upregulation on KPNA4 protein production. * P < 0.05, ** P < 0.01 and *** P < 0.001
