MAB823

antibody from R&D Systems

Targeting: CASP7 CMH-1, ICE-LAP3, MCH3

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB823

Provider

R&D Systems

Product name

Human/Mouse Caspase-7 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human and mouse precursor Caspase-7 and the large subunit of cleaved Caspase-7.

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

P55210


Isotype

IgG

Antibody clone number

MCH3101.62

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human and Mouse Caspase-7 Precursor and Large Subunit by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and DA3 mouse myeloma cell line untreated (-) or treated (+) with 1 μM staurosporine (STS) for the indicated times. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse Caspase-7 Monoclonal Antibody (Catalog # MAB823), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Caspase-7 precursor and large subunit at approximately 38 and 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.