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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- MA5-24320 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PPM1L Monoclonal Antibody (700612)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 700612
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of BJAB human Burkitt's lymphoma cell line, Nb2-11 rat lymphoma cell line, Neuro-2A mouse neuroblastoma cell line, and L1.2 mouse pro-B cell line. PVDF membrane was probed with 0.5 µg/mL of mouse Anti-human PP2C e/PPM1L Monoclonal Antibody (Product # MA5-24320) followed by HRP-conjugated Anti-mouse IgG Secondary Antibody. A specific band was detected for PP2C e/PPM1L at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PPM1L in BJAB human Burkitts lymphoma cell line, Nb2‚11 rat lymphoma cell line, Neuro‚2A mouse neuroblastoma cell line, and L1.2 mouse pro-B cell line. Samples were incubated in PPM1L monoclonal antibody (Product # MA5-24320) using a dilution of 0.5 µg/mL followed by a HRP-conjugated Anti-Mouse IgG secondary antibody. A specific band was detected for PP2C ε/PPM1L at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions.
