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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- GTX630197 - Provider product page
- Provider
- GeneTex
- Product name
- TDP43 antibody [GT733]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- TARDBP antibody [GT733] validation by siRNA knock-down.Upperpanel: TARDBP antibody [GT733] GTX630197Lower panel: GAPDH antibody (GTX100118)A. 30 £gg 293T whole cell lysate/extract B. 30 £gg whole cell lysate/extract of TARDBP shRNA-transfected 293T cells10 % SDS-PAGETARDBP antibody [GT733] (GTX630197) dilution: 1:1000GAPDH antibody (GTX100118) dilution: 1:10000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TARDBP antibody [GT733] detects TARDBP protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extract B. 30 ?g A431 whole cell lysate/extract C. 30 ?g HeLa whole cell lysate/extract D. 30 ?g HepG2 whole cell lysate/extract 10 % SDS-PAGETARDBP antibody [GT733] (GTX630197) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with TARDBP antibody [GT733] (GTX630197) diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TARDBP antibody [GT733] validation by siRNA knock-down.Upperpanel: TARDBP antibody [GT733] GTX630197Lower panel: GAPDH antibody (GTX100118)A. 30 £gg 293T whole cell lysate/extract B. 30 £gg whole cell lysate/extract of TARDBP shRNA-transfected 293T cells10 % SDS-PAGETARDBP antibody [GT733] (GTX630197) dilution: 1:1000GAPDH antibody (GTX100118) dilution: 1:10000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TARDBP antibody [GT733] detects TARDBP protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TARDBP protein stained by TARDBP antibody [GT733] (GTX630197) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TDP43 antibody [GT733] detects TDP43 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. TDP43 antibody [GT733] (GTX630197) diluted at 1:200.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TDP43 antibody [GT733] detects TDP43 protein at nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. TDP43 antibody [GT733] (GTX630197) diluted at 1:200.
