MA5-35273

antibody from Invitrogen Antibodies

Targeting: TARDBP ALS10, TDP-43

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-35273

Provider

Invitrogen Antibodies

Product name

TDP-43 Recombinant Rabbit Monoclonal Antibody (4R5L7)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Immunogen sequence: GNNQGSNMGG GMNFGAFSIN PAMMAAAQAA LQSSWGMMGM LASQQNQSGP SGNNQNQGNM QREPNQAFGS GNNSYSGSNS GAAIGWGSAS NAGSGSGFNG G

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

4R5L7

Vial size

100 µL

Concentration

0.8 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

References

Submitted references

Importin-Mediated Pathological Tau Nuclear Translocation Causes Disruption of the Nuclear Lamina, TDP-43 Mislocalization and Cell Death.

Candia RF, Cohen LS, Morozova V, Corbo C, Alonso AD
Frontiers in molecular neuroscience 2022;15:888420

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of TDP-43 performed on C6 cells using a TDP-43 monoclonal antibody (Product # MA5-35273) at a dilution of 1:100. An HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was applied at a dilution of 1:10,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of TDP-43 performed on NIH-3T3 cells using a TDP-43 monoclonal antibody (Product # MA5-35273) at a dilution of 1:100. An HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was applied at a dilution of 1:10,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of TDP-43 performed on C6 cells using a TDP-43 monoclonal antibody (Product # MA5-35273) at a dilution of 1:100. An HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was applied at a dilution of 1:10,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of TDP-43 performed on HeLa cells using a TDP-43 monoclonal antibody (Product # MA5-35273) at a dilution of 1:100. An HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was applied at a dilution of 1:10,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of TDP-43 performed on NIH-3T3 cells using a TDP-43 monoclonal antibody (Product # MA5-35273) at a dilution of 1:100. An HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was applied at a dilution of 1:10,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Confocal imaging (Immunocytochemistry) of TDP-43 in HeLa cells. Samples were incubated with TDP-43 Monoclonal antibody (Product # MA5-35273) using a dilution of 1:100 (Red). The cells were counterstained with alpha Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of TDP-43 in paraffin-embedded human esophageal. Samples were incubated with TDP-43 Monoclonal antibody (Product # MA5-35273) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of TDP-43 in paraffin-embedded mouse brain. Samples were incubated with TDP-43 Monoclonal antibody (Product # MA5-35273) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of TDP-43 in paraffin-embedded rat ovary. Samples were incubated with TDP-43 Monoclonal antibody (Product # MA5-35273) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ChIP analysis of TDP-43 in extracts of K562 cells. Samples were incubated with TDP-43 Monoclonal antibody (Product # MA5-35273) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 5 PH-tau-positive cells present mislocalization of TDP-43 to the cytoplasm when PH-tau can enter the nucleus, but not when it is kept in the cytoplasm. (A) HEK-293 cells were transfected with WT-tau, PH-tau, WT-tau-K140A,K141A, or PH-tau-K140A,K141A (green) and immunostained for TDP-43 (red). The localization of TDP-43 was determined by looking for co-localization of the TDP-43 stain and DAPI (blue), which presents as purple. The arrows indicate areas of red staining that do not co-localize with DAPI, indicating mislocalization to the cytoplasm. Scale bar indicates 15 mum. (B) Overlays of z-stack images taken with a confocal microscope. The inset displays an IMARIS reconstruction of the indicated cell to better display the nuclear or cytoplasmic localization of TDP-43 (red dots). Scale bar indicates 15 mum.