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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-78432 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SWAP70 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: Jurkat, Raji, NCI-H929, 293T, A431, HeLa, HepG2 Predicted Reactivity: Rat (95%), Chicken (89%), Rhesus Monkey (99%), Bovine (93%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SWAP70 Polyclonal Antibody detects SWAP70 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SWAP70 Polyclonal Antibody (Product # PA5-78432) diluted at a dilution of 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SWAP70 Polyclonal Antibody detects SWAP70 protein at cytoplasm by immunofluorescent analysis. Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SWAP70 protein stained by SWAP70 Polyclonal Antibody (Product # PA5-78432) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SWAP70 Polyclonal Antibody detects SWAP70 protein at cytoplasm by immunofluorescent analysis. Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SWAP70 protein stained by SWAP70 Polyclonal Antibody (Product # PA5-78432) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SWAP70 Polyclonal Antibody detects SWAP70 protein at cytosol on mouse spleen by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. SWAP70 Polyclonal Antibody (Product # PA5-78432) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
