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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [11]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB274-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-10 R alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IL-10 R alpha in direct ELISAs. In this format, this antibody does not cross-react with recombinant mouse IL-10 R alpha .
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q13651.1- Isotype
- IgG
- Antibody clone number
- 37607
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Inhibition of IL-10 and TGF-β receptors on dendritic cells enhances activation of effector T-cells to kill cholangiocarcinoma cells.
IL-10 Receptor Signaling Is Essential for TR1 Cell Function In Vivo.
Opposing regulation of the late phase TNF response by mTORC1-IL-10 signaling and hypoxia in human macrophages.
Interleukin-10 receptor-1 expression in monocyte-derived antigen-presenting cell populations: dendritic cells partially escape from IL-10's inhibitory mechanisms.
TNF-α blockade induces IL-10 expression in human CD4+ T cells.
Autocrine IL-10 promotes human B-cell differentiation into IgM- or IgG-secreting plasmablasts.
IFNγ/IL-10 co-producing cells dominate the CD4 response to malaria in highly exposed children.
Immunomodulatory properties of a viral homolog of human interleukin-10 expressed by human cytomegalovirus during the latent phase of infection.
Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function.
In vitro-expanded human CD4(+)CD25(+) T-regulatory cells can markedly inhibit allogeneic dendritic cell-stimulated MLR cultures.
Human cytomegalovirus-encoded interleukin-10 homolog inhibits maturation of dendritic cells and alters their functionality.
Thepmalee C, Panya A, Junking M, Chieochansin T, Yenchitsomanus PT
Human vaccines & immunotherapeutics 2018 Jun 3;14(6):1423-1431
Human vaccines & immunotherapeutics 2018 Jun 3;14(6):1423-1431
IL-10 Receptor Signaling Is Essential for TR1 Cell Function In Vivo.
Brockmann L, Gagliani N, Steglich B, Giannou AD, Kempski J, Pelczar P, Geffken M, Mfarrej B, Huber F, Herkel J, Wan YY, Esplugues E, Battaglia M, Krebs CF, Flavell RA, Huber S
Journal of immunology (Baltimore, Md. : 1950) 2017 Feb 1;198(3):1130-1141
Journal of immunology (Baltimore, Md. : 1950) 2017 Feb 1;198(3):1130-1141
Opposing regulation of the late phase TNF response by mTORC1-IL-10 signaling and hypoxia in human macrophages.
Huynh L, Kusnadi A, Park SH, Murata K, Park-Min KH, Ivashkiv LB
Scientific reports 2016 Aug 25;6:31959
Scientific reports 2016 Aug 25;6:31959
Interleukin-10 receptor-1 expression in monocyte-derived antigen-presenting cell populations: dendritic cells partially escape from IL-10's inhibitory mechanisms.
von Haehling S, Wolk K, Höflich C, Kunz S, Grünberg BH, Döcke WD, Reineke U, Asadullah K, Sterry W, Volk HD, Sabat R
Genes and immunity 2015 Jan-Feb;16(1):8-14
Genes and immunity 2015 Jan-Feb;16(1):8-14
TNF-α blockade induces IL-10 expression in human CD4+ T cells.
Evans HG, Roostalu U, Walter GJ, Gullick NJ, Frederiksen KS, Roberts CA, Sumner J, Baeten DL, Gerwien JG, Cope AP, Geissmann F, Kirkham BW, Taams LS
Nature communications 2014;5:3199
Nature communications 2014;5:3199
Autocrine IL-10 promotes human B-cell differentiation into IgM- or IgG-secreting plasmablasts.
Heine G, Drozdenko G, Grün JR, Chang HD, Radbruch A, Worm M
European journal of immunology 2014 Jun;44(6):1615-21
European journal of immunology 2014 Jun;44(6):1615-21
IFNγ/IL-10 co-producing cells dominate the CD4 response to malaria in highly exposed children.
Jagannathan P, Eccles-James I, Bowen K, Nankya F, Auma A, Wamala S, Ebusu C, Muhindo MK, Arinaitwe E, Briggs J, Greenhouse B, Tappero JW, Kamya MR, Dorsey G, Feeney ME
PLoS pathogens 2014 Jan;10(1):e1003864
PLoS pathogens 2014 Jan;10(1):e1003864
Immunomodulatory properties of a viral homolog of human interleukin-10 expressed by human cytomegalovirus during the latent phase of infection.
Jenkins C, Garcia W, Godwin MJ, Spencer JV, Stern JL, Abendroth A, Slobedman B
Journal of virology 2008 Apr;82(7):3736-50
Journal of virology 2008 Apr;82(7):3736-50
Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function.
Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB
Blood 2005 Jan 15;105(2):750-8
Blood 2005 Jan 15;105(2):750-8
In vitro-expanded human CD4(+)CD25(+) T-regulatory cells can markedly inhibit allogeneic dendritic cell-stimulated MLR cultures.
Godfrey WR, Ge YG, Spoden DJ, Levine BL, June CH, Blazar BR, Porter SB
Blood 2004 Jul 15;104(2):453-61
Blood 2004 Jul 15;104(2):453-61
Human cytomegalovirus-encoded interleukin-10 homolog inhibits maturation of dendritic cells and alters their functionality.
Chang WL, Baumgarth N, Yu D, Barry PA
Journal of virology 2004 Aug;78(16):8720-31
Journal of virology 2004 Aug;78(16):8720-31
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- IL-10 Inhibition of IL-1 beta secretion and Neutralization by Human IL-10 R alpha Antibody. Recombinant Human IL-10 (Catalog # 217-IL) inhibits IL-1 beta secretion in LPS-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line), as measured by the Human IL-1 beta/IL-1F2 Quantikine ELISA Kit (Catalog # DLB50). IL-1 beta secretion inhibited by Recombinant Human IL-10 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-10 R alpha Monoclonal Antibody (Catalog # MAB274). The ND50 is typically 0.03-0.1 µg/mL in the presence of LPS (0.25 ng/mL).
